Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/44409

TítuloIntegration of biomass formulations of genome-scale metabolic models with experimental data reveals universally essential cofactors in prokaryotes
Autor(es)Xavier, Joana C.
Patil, Kiran Raosaheb
Rocha, Isabel
Palavras-chaveEssential cofactors
Metabolic networks
Genome-scale models
Data2017
EditoraAcademic Press
RevistaMetabolic Engineering
CitaçãoXavier, Joana C.; Patil, Kiran Raosaheb; Rocha, Isabel, Integration of biomass formulations of genome-scale metabolic models with experimental data reveals universally essential cofactors in prokaryotes. Metabolic Engineering, 39, 200-208, 2017
Resumo(s)The composition of a cell in terms of macromolecular building blocks and other organic molecules underlies the metabolic needs and capabilities of a species. Although some core biomass components such as nucleic acids and proteins are evident for most species, the essentiality of the pool of other organic molecules, especially cofactors and prosthetic groups, is yet unclear. Here we integrate biomass compositions from 71 manually curated genome-scale models, 33 large-scale gene essentiality datasets, enzyme-cofactor association data and a vast array of publications, revealing universally essential cofactors for prokaryotic metabolism and also others that are specific for phylogenetic branches or metabolic modes. Our results revise predictions of essential genes in Klebsiella pneumoniae and identify missing biosynthetic pathways in models of Mycobacterium tuberculosis. This work provides fundamental insights into the essentiality of organic cofactors and has implications for minimal cell studies as well as for modeling genotype-phenotype relations in prokaryotic metabolic networks.
Tipoarticle
URIhttp://hdl.handle.net/1822/44409
DOI10.1016/j.ymben.2016.12.002
ISSN1096-7176
e-ISSN1096-7184
Arbitragem científicayes
AcessoopenAccess
Aparece nas coleções:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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