Please use this identifier to cite or link to this item:

TitleMultilayered hollow tubes as blood vessel substitutes
Author(s)Silva, J. M.
Custódio, Catarina Almeida
Reis, R. L.
Mano, J. F.
KeywordsBlood vessel substitutes
Endothelial cells
Smooth muscle cells
Tissue engineering
Issue dateNov-2016
PublisherAmerican Chemical Society
JournalACS Biomaterials Science and Engineering
CitationSilva J. M., Custódio C. A., Reis R. L., Mano J. F. Multilayered Hollow Tubes as Blood Vessel Substitutes, ACS biomaterials science & engineering, Vol. 12, Issue 2, pp. 2304–2314, doi:10.1021/acsbiomaterials.6b00499, 2016
Abstract(s)The available therapies for cardiovascular pathologies often require the replacement of diseased vascular grafts. However, the current blood vessel substitutes are unsuitable for small-diameter blood vessel replacements. Herein, we propose the creation of multilayered hollow tubes as blood vessel substitutes. Hollow tubes were obtained by building-up multilayers of marine-derived polysaccharides (i.e., chitosan and alginate) on sacrificial tubular templates using layer-by-layer technology and template leaching. A cross-linking degree of ≈ 59 % was achieved using genipin, which is reflected in an increase of the mechanical properties and a decrease on the water uptake. To further improve the cell adhesive properties of the multilayers, fibronectin (FN) was immobilized on the surface of the hollow tubes. In vitro biological performance of human umbilical vein endothelial cells (HUVECs) and human aortic smooth muscle cells (HASMCs) were assessed. In addition, to perform the culture of HUVECs on the inner side and the HASMCs on the outer side of the tubes, an in-house developed apparatus was created that allowed us to feed cells with their respective culture medium. The developed hollow tubes showed to be a suitable structure to promote cell adhesion, spreading, and proliferation. It is our belief, that the creation of these functional structures will open a new research field in order to develop innovative multilayered tubular structures for cardiovascular TE applications.
Publisher version
AccessRestricted access (UMinho)
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

Files in This Item:
File Description SizeFormat 
18902-silva2016 ACS.pdf
  Restricted access
6,99 MBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID