Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/44372

TitleMultilayered hollow tubes as blood vessel substitutes
Author(s)Silva, J. M.
Custódio, Catarina Almeida
Reis, R. L.
Mano, J. F.
KeywordsBlood vessel substitutes
Endothelial cells
Layer-by-layer
Nanobiomaterials
Smooth muscle cells
Tissue engineering
Issue dateNov-2016
PublisherAmerican Chemical Society
JournalACS Biomaterials Science and Engineering
CitationSilva J. M., Custódio C. A., Reis R. L., Mano J. F. Multilayered Hollow Tubes as Blood Vessel Substitutes, ACS biomaterials science & engineering, Vol. 12, Issue 2, pp. 2304–2314, doi:10.1021/acsbiomaterials.6b00499, 2016
Abstract(s)The available therapies for cardiovascular pathologies often require the replacement of diseased vascular grafts. However, the current blood vessel substitutes are unsuitable for small-diameter blood vessel replacements. Herein, we propose the creation of multilayered hollow tubes as blood vessel substitutes. Hollow tubes were obtained by building-up multilayers of marine-derived polysaccharides (i.e., chitosan and alginate) on sacrificial tubular templates using layer-by-layer technology and template leaching. A cross-linking degree of ≈ 59 % was achieved using genipin, which is reflected in an increase of the mechanical properties and a decrease on the water uptake. To further improve the cell adhesive properties of the multilayers, fibronectin (FN) was immobilized on the surface of the hollow tubes. In vitro biological performance of human umbilical vein endothelial cells (HUVECs) and human aortic smooth muscle cells (HASMCs) were assessed. In addition, to perform the culture of HUVECs on the inner side and the HASMCs on the outer side of the tubes, an in-house developed apparatus was created that allowed us to feed cells with their respective culture medium. The developed hollow tubes showed to be a suitable structure to promote cell adhesion, spreading, and proliferation. It is our belief, that the creation of these functional structures will open a new research field in order to develop innovative multilayered tubular structures for cardiovascular TE applications.
TypeArticle
URIhttp://hdl.handle.net/1822/44372
DOI10.1021/acsbiomaterials.6b00499
ISSN2373-9878
Publisher versionhttp://pubs.acs.org/doi/abs/10.1021/acsbiomaterials.6b00499
Peer-Reviewedyes
AccessRestricted access (UMinho)
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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