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TitleProtective effect of antigen delivery using monoolein-based liposomes in experimental hematogenously disseminated candidiasis
Author(s)Carneiro, Catarina
Correia, Alexandra
Lima, Tanea
Vilanova, Manuel
Pais, Célia
Gomes, Andreia
Real Oliveira, M. Elisabete C.D.
Sampaio, Paula
KeywordsDODAB:MO liposomes
antigen delivery systems
candida albicans proteins
dendritic cells
Humoral and cellular immune responses
Issue date1-May-2016
JournalActa Biomaterialia
CitationActa Biomaterialia 39 (2016) 133–145
Abstract(s)We evaluated the potential of a liposomal antigen delivery system (ADS) containing Candida albicans cell wall surface proteins (CWSP) in mediating protection against systemic candidiasis. Treatment of bonemarrow- derived dendritic cells with CWSP-loaded dioctadecyldimethylammonium bromide:monoolein (DODAB:MO) liposomes enhanced and prolonged their activation comparatively to free antigen, indicating that liposome-entrapped CWSP were released more sustainable. Therefore, we immunized mice with CWSP either in a free form or loaded into two different DODAB:MO liposome formulations, respectively designated as ADS1 and ADS2, prior to intravenous C. albicans infection. Immunization with ADS1, but not with ADS2, conferred significant protection to infected mice, comparatively to immunization with CWSP or empty liposomes as control. ADS1-immunized mice presented significantly higher serum levels of C. albicans-specific antibodies that enhanced phagocytosis of this fungus. In these mice, a mixed cytokine production profile was observed encompassing IFN-c, IL-4, IL-17A and IL-10. Nevertheless, only production of IL-4, IL-17 and IL-10 was higher than in controls. In this study we demonstrated that DODAB:MO liposomes enhance the immunogenicity of C. albicans antigens and host protection in a murine model of systemic candidiasis. Therefore, this liposomal adjuvant could be a promising candidate to assess in vaccination against this pathogenic fungus.
AccessOpen access
Appears in Collections:CDF - FAMO - Artigos/Papers (with refereeing)
DBio - Artigos/Papers

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