Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/43836

TitleQuantitative assessment of the regenerative and mineralogenic performances of the zebrafish caudal fin
Author(s)Cardeira, João
Gavaia, Paulo J.
Fernández, Ignacio
Cengiz, I. F.
Silva, Joana M.
Oliveira, J. M.
Reis, R. L.
Cancela, M. Leonor
Laizé, Vincent
KeywordsMineralogenic performance
Zebrafish
Issue dateDec-2016
PublisherNature Publishing Group
JournalScientific Reports
CitationCardeira, J., Gavaia, P. J., Fernández, I., Cengiz, I. F., Moreira-Silva, J., Oliveira, J. M., . . . Laizé, V. (2016). Quantitative assessment of the regenerative and mineralogenic performances of the zebrafish caudal fin. [Article]. Scientific Reports, 6. doi: 10.1038/srep39191
Abstract(s)The ability of zebrafish to fully regenerate its caudal fin has been explored to better understand the mechanisms underlying de novo bone formation and to develop screening methods towards the discovery of compounds with therapeutic potential. Quantifying caudal fin regeneration largely depends on successfully measuring new tissue formation through methods that require optimization and standardization. Here, we present an improved methodology to characterize and analyse overall caudal fin and bone regeneration in adult zebrafish. First, regenerated and mineralized areas are evaluated through broad, rapid and specific chronological and morphometric analysis in alizarin red stained fins. Then, following a more refined strategy, the intensity of the staining within a 2D longitudinal plane is determined through pixel intensity analysis, as an indicator of density or thickness/ volume. The applicability of this methodology on live specimens, to reduce animal experimentation and provide a tool for in vivo tracking of the regenerative process, was successfully demonstrated. Finally, the methodology was validated on retinoic acid- and warfarin-treated specimens, and further confirmed by micro-computed tomography. Because it is easily implementable, accurate and does not require sophisticated equipment, the present methodology will certainly provide valuable technical standardization for research in tissue engineering, regenerative medicine and skeletal biology.
TypeArticle
URIhttp://hdl.handle.net/1822/43836
DOI10.1038/srep39191
ISSN2045-2322
Publisher versionhttp://www.nature.com/articles/srep39191
Peer-Reviewedyes
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

Files in This Item:
File Description SizeFormat 
18900-srep39191.pdf3,05 MBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID