Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/43836

TítuloQuantitative assessment of the regenerative and mineralogenic performances of the zebrafish caudal fin
AutorCardeira, João
Gavaia, Paulo J.
Fernández, Ignacio
Cengiz, I. F.
Silva, Joana M.
Oliveira, J. M.
Reis, R. L.
Cancela, M. Leonor
Laizé, Vincent
Palavras-chaveMineralogenic performance
Zebrafish
DataDez-2016
EditoraNature Publishing Group
CitaçãoCardeira, J., Gavaia, P. J., Fernández, I., Cengiz, I. F., Moreira-Silva, J., Oliveira, J. M., . . . Laizé, V. (2016). Quantitative assessment of the regenerative and mineralogenic performances of the zebrafish caudal fin. [Article]. Scientific Reports, 6. doi: 10.1038/srep39191
ResumoThe ability of zebrafish to fully regenerate its caudal fin has been explored to better understand the mechanisms underlying de novo bone formation and to develop screening methods towards the discovery of compounds with therapeutic potential. Quantifying caudal fin regeneration largely depends on successfully measuring new tissue formation through methods that require optimization and standardization. Here, we present an improved methodology to characterize and analyse overall caudal fin and bone regeneration in adult zebrafish. First, regenerated and mineralized areas are evaluated through broad, rapid and specific chronological and morphometric analysis in alizarin red stained fins. Then, following a more refined strategy, the intensity of the staining within a 2D longitudinal plane is determined through pixel intensity analysis, as an indicator of density or thickness/ volume. The applicability of this methodology on live specimens, to reduce animal experimentation and provide a tool for in vivo tracking of the regenerative process, was successfully demonstrated. Finally, the methodology was validated on retinoic acid- and warfarin-treated specimens, and further confirmed by micro-computed tomography. Because it is easily implementable, accurate and does not require sophisticated equipment, the present methodology will certainly provide valuable technical standardization for research in tissue engineering, regenerative medicine and skeletal biology.
Tipoarticle
URIhttp://hdl.handle.net/1822/43836
DOI10.1038/srep39191
ISSN2045-2322
Versão da editorahttp://www.nature.com/articles/srep39191
Arbitragem científicayes
AcessoopenAccess
Aparece nas coleções:3B’s - Artigos em revistas/Papers in scientific journals

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