Please use this identifier to cite or link to this item:

TitleHigh-throughput topographic, mechanical, and biological screening of multilayer films containing mussel-inspired biopolymers
Author(s)Neto, Ana I.
Vasconcelos, Natália L.
Oliveira, Sara M.
Ruiz-Molina, Daniel
Mano, J. F.
KeywordsBiological screening
High throughput analysis
Layer-by-layer assembly
Mechanical screening
Mussel-Inspired Biopolymers
Topographical screening
Issue dateFeb-2016
JournalAdvanced Functional Materials
CitationNeto A. I., Vasconcelos N. L., Oliveira S. M., Ruiz-Molina D., Mano J. F. High-Throughput Topographic, Mechanical, and Biological Screening of Multilayer Films Containing Mussel-Inspired Biopolymers, Advanced Functional Materials, Vol. 26, Issue 16, pp. 2745–2755, doi:10.1002/adfm.201505047, 2016
Abstract(s)A high-content screening method to characterize multifunctional multilayer films that combine mechanical adhesion and favorable biological response is reported. Distinct combinations of nanostructured films are produced using layer-by-layer methodology and their morphological, physicochemical, and biological properties are analyzed in a single microarray chip. Inspired by the composition of the adhesive proteins in mussels, thin films containing dopamine-modified hyaluronic acid are studied. Flat biomimetic superhydrophobic patterned chips produced by a bench-top methodology are used for the build-up of arrays of multilayer films. The wettability contrasts imprinted onto the chips are allowed to produce individual, position controlled, multilayer films in the wettable regions. The flat configuration of the chip permits to perform a series of nondestructive measurements directly on the individual spots. In situ adhesion properties are directly measured in each spot, showing that nanostructured films richer in dopamine promote the adhesion. In vitro tests show an enhanced cell adhesion for the films with more catechol groups. The advantages presented by this platform include ability to control the uniformity and size of the multilayers films, its suitability to be used as a new low cost toolbox and for high-content cellular screening, and capability for monitoring in situ a variety of distinct material properties.
Publisher version
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

Files in This Item:
File Description SizeFormat 
18663-Neto_et_al-2016-Advanced_Functional_Materials.pdf8,66 MBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID