Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/43590

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dc.contributor.authorPerugini, V.por
dc.contributor.authorGuildford, A.por
dc.contributor.authorSilva-Correia, Joanapor
dc.contributor.authorOliveira, J. M.por
dc.contributor.authorMeikle, S. T.por
dc.contributor.authorReis, R. L.por
dc.contributor.authorSantin, M.por
dc.identifier.citationPerugini V., Guildford A., Silva-Correia J., Oliveira J. M., Meikle S. T., Reis R. L., Santin M. Anti-angiogenic Potential of VEGF Blocker Dendron-laden Gellan Gum Hydrogels for Tissue Engineering Applications, Journal of Tissue Engineering and Regenerative Medicine, doi:10.1002/term.2340, 2017.por
dc.description.abstractDamage of non-vascularised tissues such as cartilage and cornea can result in healing processes accompanied by a non-physiological angiogenesis. Peptidic aptamers have recently been reported to block the vascular endothelial growth factor (VEGF). However, the therapeutic applications of these aptamers is limited due to their short half-life in vivo. In this work, an enhanced stability and bioavailability of a known VEGF blocker aptamer sequence (WHLPFKC) was pursued through its tethering of molecular scaffolds based on hyperbranched peptides, the poly(É -lysine) dendrons, bearing three branching generations. The proposed design allowed simultaneous and orderly-spaced exposure of sixteen aptamers per dendrimer to the surrounding biological microenvironent, as well as a relatively hydrophobic core based on di-phenylalanine aiming to promote an hydrophobic interaction with the hydrophobic moieties of ionically-crosslinked metacrylated gellan gum (iGG-MA) hydrogels. The VEGF blocker dendrons were entrapped in iGG-MA hydrogels and their capacity to prevent endothelial cell sprouting was assessed qualitatively and quantitatively using 3D in vitro models and the in vivo chick chorioallantoic membrane (CAM) assay. The data demonstrate that at nanoscale concentrations, the dendronised structures were able to enhance control of the biological actvity of WHLPFKC at the material/tissue interface and hence the anti-angiogenic capacity of iGG-MA hydrogels not only preventing blood vessel invasion, but also inducing their regression at the tissue/iGG-MA interface. The in ovo study confirmed that iGG-MA functionalised with the dendron VEGF blockers do inhibit angiogenesis by controlling both size and ramifications of blood vessels in proximity of the implanted gel surface.por
dc.description.sponsorshipThis work was mainly supported by the EC FP7 project Disc Regeneration (Contract No NMP3-LA-2008-213904). This study was also funded by the EC FP7 Programme contract agreement no REGPOT-CT2012-316331-POLARIS.por
dc.subjectAnti-angiogenic dendronspor
dc.subjectIVD regenerationpor
dc.subjectAnti-angiogenesis peptidepor
dc.subjectGellan gumpor
dc.subjectTissue regenerationpor
dc.subjectVascular endothelial growth factorpor
dc.titleAnti-angiogenic potential of VEGF blocker dendron-laden gellan gum hydrogels for tissue engineering applicationspor
degois.publication.titleJournal of Tissue Engineering and Regenerative Medicinepor
sdum.journalJournal of Tissue Engineering and Regenerative Medicinepor
Aparece nas coleções:3B’s - Artigos em revistas/Papers in scientific journals

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