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TitleFluorescent quantification of melanin
Author(s)Fernandes, Bruno Pacheco
Matamá, Maria Teresa
Guimarães, Diana Isabel Pereira
Gomes, Andreia
Cavaco-Paulo, Artur
Pigment quantification
Melanin oxidation
Melanin fluorescence
Absorption spectroscopy
Issue date2016
PublisherJohn Wiley and Sons
JournalPigment Cell and Melanoma Research
CitationFernandes, Bruno P.; Matamá, Maria Teresa; Guimarães, D.; Gomes, Andreia; Cavaco-Paulo, Artur, Fluorescent quantification of melanin. Pigment Cell and Melanoma Research, 29(6), 707-712, 2016
Abstract(s)Melanin quantification is reportedly performed by absorption spectroscopy, commonly at 405 nm. Here, we propose the implementation of fluorescence spectroscopy for melanin assessment. In a typical in vitro assay to assess melanin production in response to an external stimulus, absorption spectroscopy clearly overvalues melanin content. This method is also incapable of distinguishing non-melanotic/amelanotic control cells from those that are actually capable of performing melanogenesis. Therefore, fluorescence spectroscopy is the best method for melanin quantification as it proved to be highly specific and accurate, detecting even small variations in the synthesis of melanin. This method can also be applied to the quantification of melanin in more complex biological matrices like zebrafish embryos and human hair.
Publisher version
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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