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|Título:||Microengineered multicomponent hydrogel fibers: combining polyelectrolyte complexation and microfluidics|
|Autor:||Almeida, Raquel Costa|
Borges, João Paulo
Babo, Pedro Miguel Sousa
Rodrigues, Márcia T.
Mano, J. F.
Reis, R. L.
Gomes, Manuela E.
|Citação:||Costa-Almeida R., Gasperini L., Borges J., Babo P. S., Rodrigues M. T., Mano J. F., Reis R. L., Gomes M. E. Microengineered Multicomponent Hydrogel Fibers: Combining Polyelectrolyte Complexation and Microfluidics, ACS Biomaterials Science & Engineering , doi:10.1021/acsbiomaterials.6b00331, 2016|
|Resumo:||Fiber-based techniques hold great potentialÂ toward the development of structures that mimic the architecture of fibrous tissues, such as tendon. MicrofluidicsÂ and polyelectrolyte complexation are among the most widelyÂ used techniques for the fabrication of fibrous structures. In thisÂ work, we combined both techniques to generate hydrogel fibersÂ with a fibrillar-like structure. For this, either methacrylatedÂ hyaluronic acid (MA-HA) or chondroitin sulfate (MA-CS) wereÂ mixed with alginate (ALG), being all negatively chargedÂ polysaccharides, combined with chitosan (CHT), which isÂ positively charged, and separately injected into a microfluidicÂ device. Through a continuous injection into a coagulation bathÂ and subsequent photo-cross-linking, we could obtain multi-component hydrogel fibers, which exhibited smaller fibrils aligned in parallel, whenever CHT was present. The biological performance was assessed upon encapsulation and further culture of tendon cells. Overall, the reported process did not affect cell viability and cells were also able to maintain their main function of producing extracellular matrix up to 21 days in culture. In summary, we developed a novel class of photo-cross-linkable multicomponent hydrogel fibers than can act as bioactive modulators of cell behavior.|
|Versão da editora:||http://pubs.acs.org/doi/abs/10.1021/acsbiomaterials.6b00331|
|Aparece nas coleções:||3B’s - Artigos em revistas/Papers in scientific journals|
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