Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/42993

TitleReinforcement of poly-L-lactic acid electrospun membranes with strontium borosilicate bioactive glasses for bone tissue engineering
Author(s)Fernandes, João S.
Gentile, Piergiorgio
Martins, Margarida Isabel Barros Coelho
Neves, N. M.
Miller, Cheryl
Crawford, Aileen
Pires, R. A.
Hatton, Paul
Reis, R. L.
KeywordsBM-MSCs
Mineralisation
Osteogenic differentiation
PLLA membranes
Strontium borosilicate glasses
Issue dateOct-2016
PublisherElsevier
JournalActa Biomaterialia
CitationFernandes J. S., Gentile P., Martins I. M., Neves N. M., Miller C., Crawford A., Pires R. A., Hatton P. V., Reis R. L. Reinforcement of Poly-L-lactic acid Electrospun Membranes with Strontium Borosilicate Bioactive Glasses for Bone Tissue Engineering, Acta Biomaterialia, Vol. 44, pp. 168-177, doi:10.1016/j.actbio.2016.08.042, 2016
Abstract(s)Herein, for the first time, we combined poly-L-lactic acid (PLLA) with a strontium borosilicate bioactive glass (BBG-Sr) using electrospinning to fabricate a composite bioactive PLLA membrane loaded with 10% (w/w) of BBG-Sr glass par- ticles (PLLA-BBG-Sr). The composites were characterised by scanning electron microscopy (SEM) and microcomputer tomography (μ-CT), and the results showed that we successfully fabricated smooth and uniform fibres (1â 3 μm in width) with a homogeneous distribution of BBG-Sr microparticles (<45 μm). Degradation studies (in phosphate buffered saline) demonstrated that the incorporation of BBG-Sr glass particles into the PLLA membranes increased their degradability and water uptake with a continuous release of cations. The addition of BBG-Sr glass particles enhanced the membraneâ s mechanical properties (69% higher Young modulus and 36% higher tensile strength). Furthermore, cellular in vitro evaluation using bone marrow-derived mesenchymal stem cells (BM-MSCs) demonstrated that PLLA-BBG-Sr mem- branes promoted the osteogenic differentiation of the cells as demonstrated by increased alkaline phosphatase activity and up-regulated osteogenic gene expression (Alpl, Sp7 and Bglap) in relation to PLLA alone. These results strongly suggest that the composite PLLA membranes reinforced with the BBG-Sr glass particles have potential as an effective biomater- ial capable of promoting bone regeneration. 
TypeArticle
URIhttp://hdl.handle.net/1822/42993
DOI10.1016/j.actbio.2016.08.042
ISSN1742-7061
Publisher versionhttp://www.sciencedirect.com/science/article/pii/S174270611630438X
Peer-Reviewedyes
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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