Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/42637

TítuloDrug-eluting biodegradable ureteral stent: new approach for urothelial tumors of upper urinary tract cancer
Autor(es)Barros, Alexandre António Antunes
Browne, S.
Oliveira, C.
Lima, Estêvão Augusto Rodrigues de
Duarte, Ana Rita C.
Healy, K.
Reis, R. L.
Palavras-chaveUreteral stents
Upper tract urothelial carcinoma
Anti-cancer drugs
T24 cell line
Biodegradable polymers
Supercritical fluid technology
Data2016
EditoraElsevier
RevistaInternational Journal of Pharmaceutics
CitaçãoBarros A. A., Browne S., Oliveira C., Lima E., Duarte A. R. C., Healy K. E., Reis R. L. Drug-eluting biodegradable ureteral stent: New approach for urothelial tumors of upper urinary tract cancer, International Journal of Pharmaceutics, Vol. 00, Issue 00, pp. 00, doi:10.1016/j.ijpharm.2016.08.061, 2016
Resumo(s)Upper urinary tract urothelial carcinoma (UTUC) accounts for 5–10% of urothelial carcinomas and is a disease that has not been widely studied as carcinoma of the bladder. To avoid the problems of conventional therapies, such as the need for frequent drug instillation due to poor drug retention, we developed a biodegradable ureteral stent (BUS) impregnated by supercritical fluid CO2 (scCO2) with the most commonly used anti-cancer drugs, namely paclitaxel, epirubicin, doxorubicin, and gemcitabine. The release kinetics of anti-cancer therapeutics from drug-eluting stents was measured in artificial urine solution (AUS). The in vitro release showed a faster release in the first 72 h for the four anti-cancer drugs, after this time a plateau was achieved and finally the stent degraded after 9 days. Regarding the amount of impregnated drugs by scCO2, gemcitabine showed the highest amount of loading (19.57 μg drug/mg polymer: 2% loaded), while the lowest amount was obtained for paclitaxel (0.067 μg drug/mg polymer: 0.01% loaded). A cancer cell line (T24) was exposed to graded concentrations (0.01–2000 ng/ml) of each drugs for 4 and 72 h to determine the sensitivities of the cells to each drug (IC50). The direct and indirect contact study of the anti-cancer biodegradable ureteral stents with the T24 and HUVEC cell lines confirmed the anti-tumoral effect of the BUS impregnated with the four anti-cancer drugs tested, reducing around 75% of the viability of the T24 cell line after 72 h and demonstrating minimal cytotoxic effect on HUVECs.
Tipoarticle
URIhttp://hdl.handle.net/1822/42637
DOI10.1016/j.ijpharm.2016.08.061
ISSN0378-5173
Versão da editorahttp://www.sciencedirect.com/science/article/pii/S037851731630816X
Arbitragem científicayes
AcessorestrictedAccess
Aparece nas coleções:ICVS - Artigos em Revistas Internacionais com Referee
3B’s - Artigos em revistas/Papers in scientific journals

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