Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/42444

TítuloMagnetically-responsive hydrogels for modulation of chondrogenic differentiation
Autor(es)Popa, Elena Geta
Santo, Vitor E.
Rodrigues, M. T.
Gomes, Manuela E.
Palavras-chavecartilage
chondrogenic commitment
Magnetic nanoparticles
magnetic tissue engineering
Stem cells
sulfated polysaccharides
DataFev-2016
EditoraMultidisciplinary Digital Publishing Institute (MDPI)
RevistaPolymers
CitaçãoPopa E. G., Santo V. E., Rodrigues M. T., Gomes M. E. Magnetically-responsive hydrogels for modulation of chondrogenic differentiation, Polymers, Vol. 8, Issue 2, pp. 28, doi:10.3390/polym8020028, 2016
Resumo(s)Magnetic nanoparticles (MNPs) are attractive tools to overcome limitations of current regenerative medicine strategies, demonstrating potential to integrate therapeutic and diagnostic functionalities in highly controlled systems. In traditional tissue engineering (TE) approaches, the MNPs association with stem cells in a three-dimensional (3D) template offers the possibility to achieve a mechano-magnetic responsive system, enabling remote control actuation. Herein, we propose to study the role of MNPs integrated in κ-carrageenan (κC) hydrogels in the cellular response of human adipose-derived stem cells (hASCs) aiming at cartilage TE applications. The results indicated that the concentration of MNPs in the κC hydrogels influences cellular behavior, tuning a positive effect on cell viability, cell content and metabolic activity of hASCs, with the most promising outcomes found in 5% MNP-κC matrices. Although hASCs laden in MNPs-free- and MNPs-κC hydrogels showed similar metabolic and proliferation levels, MNPs κC hydrogels under magnetic actuation evidenced an instructive effect on hASCs, at a gene expression level, towards chondrogenic phenotype even in basic medium cultures. Therefore, the MNPs-based systems developed in this study may contribute to advanced strategies towards cartilage-like engineered substitutes.
Tipoarticle
URIhttp://hdl.handle.net/1822/42444
DOI10.3390/polym8020028
ISSN2073-4360
Arbitragem científicayes
AcessoopenAccess
Aparece nas coleções:3B’s - Artigos em revistas/Papers in scientific journals


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