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TitleStructure and mode of action of cyclic lipopeptide pseudofactin II with divalent metal ions
Author(s)Janek, Tomasz
Rodrigues, L. R.
Gudiña, Eduardo J.
Czyznikowska, Zaneta
Pseudofactin II
Metal-ion interaction
Circular dichroism
Molecular modelling
Uropathogenic microorganisms
Issue date2016
JournalColloids and Surfaces B: Biointerfaces
CitationJanek, Tomasz; Rodrigues, Lígia R.; Gudiña, Eduardo J.; Czyznikowska, Zaneta, Structure and mode of action of cyclic lipopeptide pseudofactin II with divalent metal ions. Colloids and Surfaces B: Biointerfaces, 146, 498-506, 2016
Abstract(s)The interaction of natural lipopeptide pseudofactin II with a series of doubly charged metal cations was examined by matrixassisted laserdesorption ionizationtime of flight (MALDI-TOF) mass spectrometry and molecular modelling. The molecular modelling for metal-pseudofactin II provides information on the metalpeptide binding sites. Overall, Mg2+, Ca2+ and Zn2+ favor the association with oxygen atoms spanning the peptide backbone, whereas Cu2+ is coordinated by three nitrogens. Circular dichroism (CD) results confirmed that Zn2+ and Cu2+ can disrupt the secondary structure of pseudofactin II at high concentrations, while Ca2+ and Mg2+ did not essentially affect the structure of the lipopeptide. Interestingly, our results showed that the addition of Zn2+ and Cu2+ helped smaller micelles to form larger micellar aggregates. Since pseudofactin II binds metals, we tested whether this phenomena was somehow related to its antimicrobial activity against Staphylococcus epidermidis and Proteus mirabilis. We found that the antimicrobial effect of pseudofactin II was increased by supplementation of culture media with all tested divalent metal ions. Finally, by using Gram-positive and Gram-negative bacteria we showed that the higher antimicrobial activity of metal complexes of pseudofactin II is attributed to the disruption of the cytoplasmic membrane.
Description"Available online 28 June 2016"
Publisher version
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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