Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/42165

TítuloDevelopment of a phage cocktail to control Proteus mirabilis catheter-associated urinary tract infections
Autor(es)Melo, Luís Daniel Rodrigues
Veiga, Patrícia
Cerca, Nuno
Kropinski, Andrew M.
Almeida, Carina
Azeredo, Joana
Sillankorva, Sanna
Palavras-chaveBacteriophages
Bacteriophage therapy
Biofilms
Urinary tract infection
Proteus mirabilis
Phage cocktail
Data2016
EditoraFrontiers Media
RevistaFrontiers in Microbiology
CitaçãoMelo, L.; Veiga, P.; Cerca N; Kropinski, Andrew M.; Almeida, Carina; Azeredo, Joana; Sillankorva, Sanna, Development of a phage cocktail to control Proteus mirabilis catheter-associated urinary tract infections. Frontiers in Microbiology, 7(1024), 2016
Resumo(s)Proteus mirabilis is an enterobacterium that causes catheter-associated urinary tract infections (CAUTIs) due to its ability to colonize and form crystalline biofilms on the catheters surface. CAUTIs are very difficult to treat, since biofilm structures are highly tolerant to antibiotics. Phages have been used widely to control a diversity of bacterial species, however a limited number of phages for P. mirabilis have been isolated and studied. Here we report the isolation of two novel virulent phages, the podovirus vB_PmiP_5460 and the myovirus vB_PmiM_5461, which are able to target respectively, 16 of the 26 and all the Proteus strains tested in this study. Both phages have been characterized thoroughly and sequencing data revealed no traces of genes associated with lysogeny. To further evaluate the phages ability to prevent catheter´s colonization by Proteus, the phages adherence to silicone surfaces was assessed. Further tests in phage-coated catheters using a dynamic biofilm model simulating CAUTIs, have shown a significant reduction of P. mirabilis biofilm formation up to 168 h of catheterization. These results highlight the potential usefulness of the two isolated phages for the prevention of surface colonization by this bacterium.
Tipoarticle
Descrição"Article 1024"
URIhttp://hdl.handle.net/1822/42165
DOI10.3389/fmicb.2016.01024
ISSN1664-302X
e-ISSN1664-302X
Versão da editorahttp://journal.frontiersin.org/journal/microbiology
Arbitragem científicayes
AcessoopenAccess
Aparece nas coleções:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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