Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/42056

TitleEnhancement of fructosyltransferase and fructooligosaccharides production by A. oryzae DIA-MF in Solid-State Fermentation using aguamiel as culture medium
Author(s)Muñiz-Márquez, Diana B.
Contreras, Juan C.
Rodríguez, Raúl
Mussatto, Solange I.
Teixeira, J. A.
Aguilar, Cristóbal N.
KeywordsFructosyltransferase
Fructooligosaccharides
Aguamiel
Solid State Fermentation
Issue dateJun-2016
PublisherElsevier
JournalBioresource Technology
CitationMuñiz-Márquez, Diana B.; Contreras, Juan C.; Rodríguez, Raúl; Mussatto, Solange I.; Teixeira, J. A.; Aguilar, Cristóbal N., Enhancement of fructosyltransferase and fructooligosaccharides production by A. oryzae DIA-MF in Solid-State Fermentation using aguamiel as culture medium. Bioresource Technology, 213, 276-282, 2016
Abstract(s)The aim of this work was to improve the production of fructosyltransferase (FTase) by Solid-State Fermentation (SSF) using aguamiel (agave sap) as culture medium and Aspergillus oryzae DIA-MF as producer strain. SSF was carried out evaluating the following parameters: inoculum rate, incubation temperature, initial pH and packing density to determine the most significant factors through Box-Hunter and Hunter design. The significant factors were then further optimized using a Box-Behnken design and response surface methodology. The maximum FTase activity (1347 U/L) was obtained at 32 °C, using packing density of 0.7 g/cm3. Inoculum rate and initial pH had no significant influence on the response. FOS synthesis applying the enzyme produced by A. oryzae DIA-MF was also studied using aguamiel as substrate.
TypeArticle
URIhttp://hdl.handle.net/1822/42056
DOI10.1016/j.biortech.2016.03.022
ISSN0960-8524
e-ISSN0960-8524
Publisher versionhttp://www.journals.elsevier.com/bioresource-technology/
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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