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TitleInvestigation of Dendrimer-based nanoparticles cellular uptake and cell tracking in a semiautomated microfluidic platform
Author(s)Carvalho, Mariana Rodrigues
Maia, F. Raquel
Reis, R. L.
Oliveira, J. M.
Extracellular matrix
Issue date2016
CitationCarvalho M. R., Maia F. R., Reis R. L., Oliveira J. M. Investigation of Dendrimer-based nanoparticles cellular uptake and cell tracking in a semi-automated microfluidic platform, Frontiers, doi:10.3389, 2016
Abstract(s)A microfluidic device such as Kima Pump and Vena8 biochip is able to realize functions that are not easily imaginable in conventional biological analysis, such as highly parallel, sophisticated high-throughput analysis and single-cell analysis in a well-defined manner [1]. Cancer cell tracking within the microfluidic model will be achieved by grafting fluorescent label probe Fluorescein-5(6)-isothiocyanate (FITC) to dendrimer nanoparticles allowing cell visualization by immunofluorescent staining followed by fluorescence microscopy. In this study, synthesis and physicochemical characterization of Carboxymethyl-chitosan/poly(amidoamine) dendrimer nanoparticles (CMCht/PAMAM NPâ s) were performed[2].  Several cancer cell lines such as a HeLA (cervical carcinoma cell line), HTTC-116 (Colon Carcinoma) and Glioblastome cell line (GBM) were exposed to different concentrations of CMCht/PAMAM dendrimer nanoparticles over a period of 7d. After finding the adequate NP concentration, the internalization efficiency was tested, as well as cellular trafficking, in static and dynamic conditions (Kima Pump bioreactor).
Publisher version
AccessOpen access
Appears in Collections:3B’s - Resumos em livros de atas de conferências - indexados no ISI Web of Science

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