Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/41899

TitleRAPD and SCAR markers as potential tools for detection of milk origin in dairy products: adulterant sheep breeds in Serra da Estrela cheese production
Author(s)Cunha, Joana T.
Ribeiro, Tânia I. B.
Rocha, João B.
Nunes, João
Teixeira, J. A.
Domingues, Lucília
KeywordsRAPD
SCAR
Ovine breeds
Milk adulteration
Serra da Estrela cheese
Quality control
Issue date2016
PublisherElsevier B.V.
JournalFood Chemistry
CitationCunha, Joana P.; Ribeiro, Tânia I. B.; Rocha, João B.; Nunes, João; Teixeira, J. A.; Domingues, Lucília, RAPD and SCAR markers as potential tools for detection of milk origin in dairy products: Adulterant sheep breeds in Serra da Estrela cheese production. Food Chemistry, 211, 631-636, 2016
Abstract(s)Serra da Estrela Protected Designation of Origin (PDO) cheese is the most famous Portuguese cheese and has a high commercial value. However, the adulteration of production with cheaper/lower-quality milks from non-autochthones ovine breeds compromises the quality of the final product and undervalues the original PDO cheese. A Randomly Amplified Polymorphic DNA (RAPD) method was developed for efficient detection of adulterant breeds in milk mixtures used for fraudulent production of this cheese. Furthermore, Sequence Characterized Amplified Region (SCAR) markers were designed envisioning the detection of milk adulteration in processed dairy foods. The RAPD-SCAR technique is here described, for the first time, to be potentially useful for detection of milk origin in dairy products. In this sense, our findings will play an important role on the valorization of Serra da Estrela cheese, as well as on other high-quality dairy products prone to adulteration, contributing to the further development of the dairy industry.
TypeArticle
DescriptionAvailable online 17 May 2016
URIhttp://hdl.handle.net/1822/41899
DOI10.1016/j.foodchem.2016.05.109
ISSN0308-8146
e-ISSN0308-8146
Publisher versionhttp://www.elsevier.com/locate/issn/03088146
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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