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TitleBSA/HSA ratio modulates the properties of Ca2+-induced cold gelation scaffolds
Author(s)Ribeiro, Artur J.
Volkov, Vadim
Oliveira, Mariana Braga
Padrão, Jorge
Mano, J. F.
Gomes, Andreia
Cavaco-Paulo, Artur
KeywordsTunable scaffolds
Hydrophobic/Hydrophilic ratio
Induced cold gelation
Surface wettability
Mechanical properties
Cell proliferation
Issue date2016
JournalInternational Journal of Biological Macromolecules
CitationRibeiro, Artur J.; Volkov, V.; Oliveira, Mariana B.; Padrão, Jorge; Mano, João F.; Gomes, Andreia C.; Cavaco-Paulo, Artur, BSA/HSA ratio modulates the properties of Ca2+-induced cold gelation scaffolds. International Journal of Biological Macromolecules, 89, 535-544, 2016
Abstract(s)An effective tissue engineering approach requires adjustment according to the target tissue to be engineered. The possibility of obtaining a protein-based formulation for the development of multivalent tunable scaffolds that can be adapted for several types of cells and tissues is explored in this work. The incremental substitution of bovine serum albumin (BSA) by human serum albumin (HSA), changing the scaffolds hydrophilic/hydrophobic ratio, on a previously optimized scaffold formulation resulted in a set of uniform porous scaffolds with different physical properties and associated cell proliferation profile along time. There was a general trend towards an increase in hydrophilicity, swelling degree and in vitro degradation of the scaffolds with increasing replacement of BSA by HAS. The set of BSA/HSA scaffolds presented distinct values for the storage (elastic) modulus and loss factor which were similar to those described for different native tissues such as bone, cartilage, muscle, skin and neural tissue. The preferential adhesion and proliferation of skin fibroblasts on the BSA25%HSA75% and HSA100% scaffolds, as predicted by their viscoelastic properties, demonstrate that the BSA/HSA scaffold formulation is promising for the development of scaffolds that can be tuned according to the tissue to be repaired and restored.
Description"Available online 6 May 2016"
Publisher version
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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