Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/41647

TitleCryopreservation of Cell Sheets of Adipose Stem Cells: Limitations and Successes
Author(s)Prata, F. P.
Cerqueira, M. T.
Moreira-Silva, J.
Pirraco, Rogério P.
Reis, R. L.
Marques, A. P.
Keywordsregenerative medicine
Tissue Culture
Tissue engineering
Issue dateDec-2014
PublisherMary Ann Liebert
JournalTissue Engineering. Part A
CitationPrata F. P., Cerqueira M. T., Moreira-Silva J., Pirraco R. P., Reis R. L., Marques A. P. Cryopreservation of Cell Sheets of Adipose Stem Cells: Limitations and Successes, Tissue Engineering Part A, Vol. 20, pp. S117-S117, doi: 10.1089/ten.tea.2014.5000.abstracts, 2014
Abstract(s)Cell Sheets of hASCs (hASCs-CS) have been previously proposed for wound healing applications(1, 2) and despite the concern for production time reduction, the possibility of having these hASCs-CS off-the-shelf is appealing. The goal of this work was to define a cryopreservation methodology allowing to preserve cells viability and the properties CS matrix. hASCs-CS obtained from three different donors were created in UP-cell thermoresponsive dishes(Nunc, Germany) as previously reported(1,2). Different cryopreservation conditions were considered: i)FBS plus DMSO(5% and10%); ii)0.4M of Trehalose plus DMSO (5% and 10%); iii)cryosolution PLL (Akron Biotech, USA); and iv)vitrification. The cryopreservation effect was first assessed for cellular viability by flow cytometry using 7-AAD, and after dissociating the hASCs-CS with collagenase and trypsin-EDTA 0.25%. The expression (RT-PCR) and deposition (western blot and immunocytochemistry) of collagen type I, laminin and fibronectin, and the organization (TEM) of the extracellular matrix was further assessed before and after hASCs-CS cryopreservation to determine a potential effect of the method over matrix composition and integrity. The obtained results confirmed that cell viability is affected by the cryopreservation methodology, as shown before for different CS(3). Interestingly, the matrix properties were not significantly altered and the typical cell sheetâ s easiness of manipulation for transplantation was not lost.
TypeAbstract
URIhttp://hdl.handle.net/1822/41647
ISSN1937-3341
Publisher versionhttp://online.liebertpub.com/doi/pdfplus/10.1089/ten.tea.2014.5000.abstracts
Peer-Reviewedyes
AccessOpen access
Appears in Collections:3B’s - Resumos em livros de atas de conferências - indexados no ISI Web of Science

Files in This Item:
File Description SizeFormat 
18351-Abstract_TERMIS_AM_2014.pdf1,66 MBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID