Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/41564

TitleFISHji: new ImageJ macros for the quantification of fluorescence in epifluorescence images
Author(s)Fontenete, S.
Carvalho, D.
Lourenço, Anália
Guimarães, N.
Madureira, P.
Figueiredo, C.
Azevedo, N. F.
KeywordsBiomedical
RNA
LNA
Image Analysis
Optimisation
computational image processing
Issue date15-Aug-2016
PublisherElsevier
JournalBiochemical Engineering Journal
CitationFontenete, S.; Carvalho, D.; Lourenço, Anália; Guimarães, N.; Madureira, P.; Figueiredo, C.; Azevedo, N. F., FISHji: new ImageJ macros for the quantification of fluorescence in epifluorescence images. Biochemical Engineering Journal, 112, 61-69, 2016
Abstract(s)Fluorescence in situ hybridization (FISH) is based on the use of fluorescent staining dyes, however, the signal intensity of the images obtained by microscopy is seldom quantified with accuracy by the researcher. The development of innovative digital image processing programs and tools has been trying to overcome this problem, however, the determination of fluorescent intensity in microscopy images still has issues due to the lack of precision in the results and the complexity of existing software. This work presents FISHji, a set of new ImageJ methods for automated quantification of fluorescence in images obtained by epifluorescence microscopy. To validate the methods, results obtained by FISHji were compared with results obtained by flow cytometry. The mean correlation between FISHji and flow cytometry was high and significant, showing that the imaging methods are able to accurately assess the signal intensity of fluorescence images. FISHji are available for non-commercial use at http://paginas.fe.up.pt/nazevedo/.
TypeArticle
URIhttp://hdl.handle.net/1822/41564
DOI10.1016/j.bej.2016.04.001
ISSN1369-703X
e-ISSN1369-703X
Publisher versionhttp://www.elsevier.com/locate/issn/1369703X
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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