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|Title:||Compositional analysis of biofilms formed by Staphylococcus aureus isolated from food sources|
Nicolau, Anca I.
|Journal:||Frontiers in Microbiology|
|Citation:||Oniciuc, Elena-Alexandra; Cerca N; Nicolau, Anca I., Compositional analysis of biofilms formed by Staphylococcus aureus isolated from food sources. Frontiers in Microbiology, 7(370), 1-4, 2016|
|Abstract(s):||Sixteen Staphylococcus aureus isolatesoriginatingfromfoods(eightfromdairyproducts, ﬁve from ﬁsh and ﬁsh products and three from meat and meat products) were evaluated regarding their bioﬁlms formation ability. Six strains (E2, E6, E8, E10, E16, and E23) distinguished as strong bioﬁlm formers, either in standard Tryptic Soy Broth or in Tryptic Soy Broth supplemented with 0.4% glucose or with 4% NaCl. The composition of the bioﬁlms formed by these S. aureus strains on polystyrene surfaces was ﬁrst inferred using enzymatic and chemical treatments. Later on, bioﬁlms were characterized by confocal laser scanning microscope (CLSM). Our experiments proved that protein-based matrices are of prime importance for the structure of bioﬁlms formed by S. aureus strains isolated from food sources. These bioﬁlm matrix compositions are similar to those put into evidence for coagulase negative staphylococci. This is a new ﬁnding having in view that scientiﬁc literature mentions exopolysaccharide abundance in bioﬁlms produced by clinical isolates and food processing environment isolates of S. aureus.|
|Description:||The Supplementary Material for this article can be found online at: http://journal.frontiersin.org/article/10.3389/fmicb. 2016.00390|
|Appears in Collections:||CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series|