Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/41538

TitleCompositional analysis of biofilms formed by Staphylococcus aureus isolated from food sources
Author(s)Oniciuc, Elena-Alexandra
Cerca, Nuno
Nicolau, Anca I.
KeywordsStaphylococcus aureus
biofilm
food
CLSM
exopolysaccharide
protein
biofilm
Issue date30-Mar-2016
PublisherFrontiers Media
JournalFrontiers in Microbiology
CitationOniciuc, Elena-Alexandra; Cerca N; Nicolau, Anca I., Compositional analysis of biofilms formed by Staphylococcus aureus isolated from food sources. Frontiers in Microbiology, 7(370), 1-4, 2016
Abstract(s)Sixteen Staphylococcus aureus isolatesoriginatingfromfoods(eightfromdairyproducts, five from fish and fish products and three from meat and meat products) were evaluated regarding their biofilms formation ability. Six strains (E2, E6, E8, E10, E16, and E23) distinguished as strong biofilm formers, either in standard Tryptic Soy Broth or in Tryptic Soy Broth supplemented with 0.4% glucose or with 4% NaCl. The composition of the biofilms formed by these S. aureus strains on polystyrene surfaces was first inferred using enzymatic and chemical treatments. Later on, biofilms were characterized by confocal laser scanning microscope (CLSM). Our experiments proved that protein-based matrices are of prime importance for the structure of biofilms formed by S. aureus strains isolated from food sources. These biofilm matrix compositions are similar to those put into evidence for coagulase negative staphylococci. This is a new finding having in view that scientific literature mentions exopolysaccharide abundance in biofilms produced by clinical isolates and food processing environment isolates of S. aureus.
TypeArticle
DescriptionThe Supplementary Material for this article can be found online at: http://journal.frontiersin.org/article/10.3389/fmicb. 2016.00390
URIhttp://hdl.handle.net/1822/41538
DOI10.3389/fmicb.7016.00390
ISSN1664-302X
e-ISSN1664-302X
Publisher versionhttp://journal.frontiersin.org/journal/microbiology
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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