Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/41411

TitleStructural and enzymatic characterization of ABgp46, a novel phage endolysin with broad anti-Gram-negative bacterial activity
Author(s)Oliveira, Hugo Alexandre Mendes
Boas, Diana Patrícia Andrade Vilas
Mesnage, Stéphane
Kluskens, Leon
Lavigne, Rob
Sillankorva, Sanna
Secundo, Francesco
Azeredo, Joana
KeywordsAcinetobacter baumannii
Phage endolysins
Mass spectrometry
Circular dichroism
Antibacterial activity
Issue dateFeb-2016
PublisherFrontiers Media
JournalFrontiers in Microbiology
CitationOliveira, Hugo; Vilas Boas, Diana; Mesnage, Stéphane; Kluskens, Leon; Lavigne, R.; Sillankorva, Sanna; Secundo, Francesco; Azeredo, Joana, Structural and enzymatic characterization of ABgp46, a novel phage endolysin with broad anti-Gram-negative bacterial activity. Frontiers in Microbiology, 7(208), 1-9, 2016
Abstract(s)The present study demonstrates the antibacterial potential of a phage endolysin against Gram-negative pathogens, particularly against multidrug resistant strains of Acinetobacter baumannii. We have cloned, heterologously expressed and characterized a novel endolysin (ABgp46) from Acinetobacter phage vb_AbaP_CEB1 and tested its antibacterial activity against several multidrug-resistant A. baumannii strains. LC-MS revealed that ABgp46 is an N-acetylmuramidase, that is also active over a broad pH range (4.0-10.0) and temperatures up to 50°C. Interestingly, ABgp46 has intrinsic and specific anti-A. baumannii activity, reducing multidrug resistant strains by up to 2 logs within 2 hours. By combining ABgp46 with several organic acids that act as outer membrane permeabilizing agents, it is possible to increase and broaden antibacterial activity to include other Gram-negative bacterial pathogens. In the presence of citric and malic acid, ABgp46 reduces A. baumannii below the detection limit (> 5 log) and more than 4 logs P. aeruginosa and Salmonella Typhimurium strains. Overall, this globular endolysin exhibits a broad and high activity against Gram-negative pathogens, that can be enhanced in presence of citric and malic acid, and be used in human and veterinary medicine.
TypeArticle
URIhttp://hdl.handle.net/1822/41411
DOI10.3389/fmicb.2016.00208
ISSN1664-302X
e-ISSN1664-302X
Publisher versionhttp://journal.frontiersin.org/article/10.3389/fmicb.2016.00208/full
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

Files in This Item:
File Description SizeFormat 
document_37838_1.pdf429,04 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID