Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/4135
Título: | The protective effect of Glutaraldehyde in the extraction of EPS from biofilms |
Autor(es): | Azeredo, Joana Henriques, Mariana Sillankorva, Sanna Oliveira, Rosário |
Palavras-chave: | Biofilm Extraction methods Glutaraldehyde ATP Cell lysis |
Data: | 2001 |
Citação: | AZEREDO, Joana [et al.] – “The protective effect of Glutaraldehyde in the extraction of EPS from biofilms”. In INTERNATIONAL SPECIALISED CONFERENCE ON MICROORGANISMS IN ACTIVATED SLUDGE AND BIOFILM, 3, Rome, 2001 – “Proceedings 3rd IWA international specialised conference on microorganisms in activated sludge and biofilm processes, Rome, Italy, June 13-15 2001” [CD-ROM]. Roma : [s.n], 2001. |
Resumo(s): | The extraction of the exopolymeric matrix is a prerequisite to properly study the composition of the biofilm. Several extraction methods were already developed, however no universal method has yet been adopted because the compromise between high yields of extraction and minimum cell lysis is difficult to establish. In fact, most of the extraction methods promote leakage of intracellular material. The most common extraction methods, Dowex resin and sonication, where assayed in biofilms of Pseudomonas fluorescens and Alcaligenes denitrificans submitted to a pre-treatment with glutaraldehyde (GTA). The assessment of ATP released after extraction was used as a criterion of cell lysis. The results showed that GTA is a protective agent against cell lysis. The pre-treatment with GTA is particularly useful combined with sonication. |
Tipo: | Artigo em ata de conferência |
URI: | https://hdl.handle.net/1822/4135 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Artigos em Livros de Atas / Papers in Proceedings |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Roma-005[1].PDF | 25,1 kB | Adobe PDF | Ver/Abrir |