Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/40943

TítuloGellan gum-hyaluronate spongy-like hydrogels promote angiogenesis in hindlimb ischemia
Autor(es)Silva, Lucília Pereira
Cerqueira, Mariana Teixeira
Pirraco, Rogério P.
Santos, T. C.
Reis, R. L.
Correlo, V. M.
Marques, A. P.
Palavras-chaveAngiogenesis
Hindlimb ischemia
Hyaluronic acid
Hydrogel
DataSet-2015
EditoraMary Ann Liebert
RevistaTissue Engineering. Part A
Citaçãoda Silva L. P., Cerqueira M. T., Pirraco R. P., Santos T. C., Reis R. L., Correlo V. M., Marques A. P. Gellan Gum-Hyaluronate Spongy-like Hydrogels Promote Angiogenesis in Hindlimb Ischemia., 2015 4th TERMIS World Congress , doi:10.1089/ten.tea.2015.5000.abstracts, 2015
Resumo(s)Gellan gum (GG) spongy-like hydrogels are highly attractive materials for Tissue Engineering applications once they present hydrogel main properties and are capable to promote cell adhesion. Considering the inefficient vascularization of thick 3D constructs, we hypothesised that GG spongy-like hydrogels containing hyaluronate (HA) would promote the recruitment of endothelial cells upon degradation, due to the angiogenic nature of the released HA fragments. GGHA spongy-like hydrogels (1% and 2%, w/v) were prepared as previously described1,2. The susceptibility of the materials to hyaluronidase degradation was demonstrated in vitro by the increased amount of reducing sugars (DNS assay) and low molecular weight HA fragments released up to 28 days (GPC). After implantation in an ischemia hindlimb mouse model, increased blood perfusion, in comparison to the control, was observed in the presence of GGHA materials. Furthermore, blood vessels density was higher in the presence of the materials, 1% (5.5±1.5) and 2% (6.0±1.6), relatively to the control (4.5±1.5). To understand vascular network maturation and stability, α-SMA positive vessels were quantified and showed a higher percentage in the presence of GGHA 1%. Based on the degradation results these differences seem to be related to the faster GGHA 1% degradation rate. GGHA spongy-like hydrogels, combining specific intrinsic features with the biorecognition of HA, are potential materials to be used in acellular and cellular approaches to improve neotissue vascularization.
TipoconferenceAbstract
Descrição"Tissue engineering: part A", vol. 21, suppl. 1 (2015)
URIhttp://hdl.handle.net/1822/40943
ISSN2152-4947
Arbitragem científicayes
AcessoopenAccess
Aparece nas coleções:3B’s - Resumos em livros de atas de conferências - indexados no ISI Web of Science

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