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dc.contributor.authorCorreia, Clara R.por
dc.contributor.authorPirraco, Rogério P.por
dc.contributor.authorCerqueira, Mariana Teixeirapor
dc.contributor.authorMarques, A. P.por
dc.contributor.authorReis, R. L.por
dc.contributor.authorMano, J. F.por
dc.identifier.citationCorreia C. R., Pirraco R. P., Cerqueira M. T., Marques A. P., Reis R. L., Mano J. F. Semipermeable Capsules Wrapping a Multifunctional and Self-regulated Co-culture Microenvironment for Osteogenic Differentiation, Scientific Reports, Vol. 6, pp. 21883, doi:10.1038/srep21883, 2016por
dc.description.abstractA new concept of semipermeable reservoirs containing co-cultures of cells and supporting microparticles is presented, inspired by the multi-phenotypic cellular environment of bone. Based on the deconstruction of the â stem cell nicheâ , the developed capsules are designed to drive a self-regulated osteogenesis. PLLA microparticles functionalized with collagen I, and a co-culture of adipose stem (ASCs) and endothelial (ECs) cells are immobilized in spherical liquified capsules. The capsules are coated with multilayers of poly(L-lysine), alginate, and chitosan nano-assembled through layer-by-layer. Capsules encapsulating ASCs alone or in a co-culture with ECs are cultured in endothelial medium with or without osteogenic differentiation factors. Results show that osteogenesis is enhanced by the co-encapsulation, which occurs even in the absence of differentiation factors. These findings are supported by an increased ALP activity and matrix mineralization, osteopontin detection, and the up regulation of BMP-2, RUNX2 and BSP. The liquified co-capsules also act as a VEGF and BMP-2 cytokines release system. The proposed liquified capsules might be a valuable injectable self-regulated system for bone regeneration employing highly translational cell sources.por
dc.description.sponsorshipThe authors acknowledge the financial support by the Portuguese Foundation for Science and Technology (FCT) through the Ph.D. (SFRH/BD/69529/2010-Clara R. Correia) and the Post-doc grants (SFRH/BPD/96611/2013- Mariana T. Cerqueira), and the funding of RL3-TECT-NORTE-01-0124-FEDER-000020 for Rogério P. Pirraco. This work was also supported by European Research Council grant agreement ERC-2014-ADG-669858 for project ATLAS.por
dc.publisherNature Publishing Grouppor
dc.subjectBioinspired materialspor
dc.subjectBiomedical engineeringpor
dc.subjectGrowth factor signallingpor
dc.subjectStem-cell researchpor
dc.titleSemipermeable capsules wrapping a multifunctional and self-regulated co-culture microenvironment for osteogenic differentiationpor
oaire.citationTitleScientific Reportspor
dc.subject.wosScience & Technologypor
sdum.journalScientific Reportspor
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