Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/40913
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Correia, Clara R. | por |
dc.contributor.author | Pirraco, Rogério P. | por |
dc.contributor.author | Cerqueira, Mariana Teixeira | por |
dc.contributor.author | Marques, A. P. | por |
dc.contributor.author | Reis, R. L. | por |
dc.contributor.author | Mano, J. F. | por |
dc.date.accessioned | 2016-03-23T16:22:17Z | - |
dc.date.available | 2016-03-23T16:22:17Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | Correia C. R., Pirraco R. P., Cerqueira M. T., Marques A. P., Reis R. L., Mano J. F. Semipermeable Capsules Wrapping a Multifunctional and Self-regulated Co-culture Microenvironment for Osteogenic Differentiation, Scientific Reports, Vol. 6, pp. 21883, doi:10.1038/srep21883, 2016 | por |
dc.identifier.issn | 2045-2322 | por |
dc.identifier.uri | https://hdl.handle.net/1822/40913 | - |
dc.description.abstract | A new concept of semipermeable reservoirs containing co-cultures of cells and supporting microparticles is presented, inspired by the multi-phenotypic cellular environment of bone. Based on the deconstruction of the â stem cell nicheâ , the developed capsules are designed to drive a self-regulated osteogenesis. PLLA microparticles functionalized with collagen I, and a co-culture of adipose stem (ASCs) and endothelial (ECs) cells are immobilized in spherical liquified capsules. The capsules are coated with multilayers of poly(L-lysine), alginate, and chitosan nano-assembled through layer-by-layer. Capsules encapsulating ASCs alone or in a co-culture with ECs are cultured in endothelial medium with or without osteogenic differentiation factors. Results show that osteogenesis is enhanced by the co-encapsulation, which occurs even in the absence of differentiation factors. These findings are supported by an increased ALP activity and matrix mineralization, osteopontin detection, and the up regulation of BMP-2, RUNX2 and BSP. The liquified co-capsules also act as a VEGF and BMP-2 cytokines release system. The proposed liquified capsules might be a valuable injectable self-regulated system for bone regeneration employing highly translational cell sources. | por |
dc.description.sponsorship | The authors acknowledge the financial support by the Portuguese Foundation for Science and Technology (FCT) through the Ph.D. (SFRH/BD/69529/2010-Clara R. Correia) and the Post-doc grants (SFRH/BPD/96611/2013- Mariana T. Cerqueira), and the funding of RL3-TECT-NORTE-01-0124-FEDER-000020 for Rogério P. Pirraco. This work was also supported by European Research Council grant agreement ERC-2014-ADG-669858 for project ATLAS. | por |
dc.language.iso | eng | por |
dc.publisher | Nature Publishing Group | por |
dc.relation | info:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBD%2F69529%2F2010/PT | - |
dc.relation | info:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBPD%2F96611%2F2013/PT | - |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/669858/EU | - |
dc.rights | openAccess | por |
dc.subject | Bioinspired materials | por |
dc.subject | Biomedical engineering | por |
dc.subject | Biomineralization | por |
dc.subject | Growth factor signalling | por |
dc.subject | Stem-cell research | por |
dc.title | Semipermeable capsules wrapping a multifunctional and self-regulated co-culture microenvironment for osteogenic differentiation | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.nature.com/articles/srep21883 | por |
dc.comments | http://3bs.uminho.pt/node/18675 | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 21883-1 | por |
oaire.citationEndPage | 21883-12 | por |
oaire.citationTitle | Scientific Reports | por |
oaire.citationVolume | 6 | por |
dc.date.updated | 2016-03-03T15:43:58Z | - |
dc.identifier.doi | 10.1038/srep21883 | por |
dc.identifier.pmid | 26905619 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Scientific Reports | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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18675-Artigo.pdf | 1,63 MB | Adobe PDF | Ver/Abrir |