Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/40913

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dc.contributor.authorCorreia, Clara R.por
dc.contributor.authorPirraco, Rogério P.por
dc.contributor.authorCerqueira, Mariana Teixeirapor
dc.contributor.authorMarques, A. P.por
dc.contributor.authorReis, R. L.por
dc.contributor.authorMano, J. F.por
dc.date.accessioned2016-03-23T16:22:17Z-
dc.date.available2016-03-23T16:22:17Z-
dc.date.issued2016-
dc.identifier.citationCorreia C. R., Pirraco R. P., Cerqueira M. T., Marques A. P., Reis R. L., Mano J. F. Semipermeable Capsules Wrapping a Multifunctional and Self-regulated Co-culture Microenvironment for Osteogenic Differentiation, Scientific Reports, Vol. 6, pp. 21883, doi:10.1038/srep21883, 2016por
dc.identifier.issn2045-2322por
dc.identifier.urihttp://hdl.handle.net/1822/40913-
dc.description.abstractA new concept of semipermeable reservoirs containing co-cultures of cells and supporting microparticles is presented, inspired by the multi-phenotypic cellular environment of bone. Based on the deconstruction of the â stem cell nicheâ , the developed capsules are designed to drive a self-regulated osteogenesis. PLLA microparticles functionalized with collagen I, and a co-culture of adipose stem (ASCs) and endothelial (ECs) cells are immobilized in spherical liquified capsules. The capsules are coated with multilayers of poly(L-lysine), alginate, and chitosan nano-assembled through layer-by-layer. Capsules encapsulating ASCs alone or in a co-culture with ECs are cultured in endothelial medium with or without osteogenic differentiation factors. Results show that osteogenesis is enhanced by the co-encapsulation, which occurs even in the absence of differentiation factors. These findings are supported by an increased ALP activity and matrix mineralization, osteopontin detection, and the up regulation of BMP-2, RUNX2 and BSP. The liquified co-capsules also act as a VEGF and BMP-2 cytokines release system. The proposed liquified capsules might be a valuable injectable self-regulated system for bone regeneration employing highly translational cell sources.por
dc.description.sponsorshipThe authors acknowledge the financial support by the Portuguese Foundation for Science and Technology (FCT) through the Ph.D. (SFRH/BD/69529/2010-Clara R. Correia) and the Post-doc grants (SFRH/BPD/96611/2013- Mariana T. Cerqueira), and the funding of RL3-TECT-NORTE-01-0124-FEDER-000020 for Rogério P. Pirraco. This work was also supported by European Research Council grant agreement ERC-2014-ADG-669858 for project ATLAS.por
dc.language.isoengpor
dc.publisherNature Publishing Grouppor
dc.rightsopenAccesspor
dc.subjectBioinspired materialspor
dc.subjectBiomedical engineeringpor
dc.subjectBiomineralizationpor
dc.subjectGrowth factor signallingpor
dc.subjectStem-cell researchpor
dc.titleSemipermeable capsules wrapping a multifunctional and self-regulated co-culture microenvironment for osteogenic differentiationpor
dc.typearticle-
dc.peerreviewedyespor
dc.relation.publisherversionhttp://www.nature.com/articles/srep21883por
dc.commentshttp://3bs.uminho.pt/node/18675por
sdum.publicationstatuspublishedpor
oaire.citationStartPage21883-1por
oaire.citationEndPage21883-12por
oaire.citationTitleScientific Reportspor
oaire.citationVolume6por
dc.date.updated2016-03-03T15:43:58Z-
dc.identifier.doi10.1038/srep21883por
dc.identifier.pmid26905619por
dc.subject.wosScience & Technologypor
sdum.journalScientific Reportspor
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