Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/40913

TitleSemipermeable capsules wrapping a multifunctional and self-regulated co-culture microenvironment for osteogenic differentiation
Author(s)Correia, Clara R.
Pirraco, Rogério P.
Cerqueira, Mariana Teixeira
Marques, A. P.
Reis, R. L.
Mano, J. F.
KeywordsBioinspired materials
Biomedical engineering
Biomineralization
Growth factor signalling
Stem-cell research
Issue date2016
PublisherNature Publishing Group
JournalScientific Reports
CitationCorreia C. R., Pirraco R. P., Cerqueira M. T., Marques A. P., Reis R. L., Mano J. F. Semipermeable Capsules Wrapping a Multifunctional and Self-regulated Co-culture Microenvironment for Osteogenic Differentiation, Scientific Reports, Vol. 6, pp. 21883, doi:10.1038/srep21883, 2016
Abstract(s)A new concept of semipermeable reservoirs containing co-cultures of cells and supporting microparticles is presented, inspired by the multi-phenotypic cellular environment of bone. Based on the deconstruction of the â stem cell nicheâ , the developed capsules are designed to drive a self-regulated osteogenesis. PLLA microparticles functionalized with collagen I, and a co-culture of adipose stem (ASCs) and endothelial (ECs) cells are immobilized in spherical liquified capsules. The capsules are coated with multilayers of poly(L-lysine), alginate, and chitosan nano-assembled through layer-by-layer. Capsules encapsulating ASCs alone or in a co-culture with ECs are cultured in endothelial medium with or without osteogenic differentiation factors. Results show that osteogenesis is enhanced by the co-encapsulation, which occurs even in the absence of differentiation factors. These findings are supported by an increased ALP activity and matrix mineralization, osteopontin detection, and the up regulation of BMP-2, RUNX2 and BSP. The liquified co-capsules also act as a VEGF and BMP-2 cytokines release system. The proposed liquified capsules might be a valuable injectable self-regulated system for bone regeneration employing highly translational cell sources.
TypeArticle
URIhttp://hdl.handle.net/1822/40913
DOI10.1038/srep21883
ISSN2045-2322
Publisher versionhttp://www.nature.com/articles/srep21883
Peer-Reviewedyes
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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