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|Title:||Semipermeable capsules wrapping a multifunctional and self-regulated co-culture microenvironment for osteogenic differentiation|
|Author(s):||Correia, Clara R.|
Pirraco, Rogério P.
Cerqueira, Mariana Teixeira
Marques, A. P.
Reis, R. L.
Mano, J. F.
Growth factor signalling
|Publisher:||Nature Publishing Group|
|Citation:||Correia C. R., Pirraco R. P., Cerqueira M. T., Marques A. P., Reis R. L., Mano J. F. Semipermeable Capsules Wrapping a Multifunctional and Self-regulated Co-culture Microenvironment for Osteogenic Differentiation, Scientific Reports, Vol. 6, pp. 21883, doi:10.1038/srep21883, 2016|
|Abstract(s):||A new concept of semipermeable reservoirs containing co-cultures of cells and supporting microparticles is presented, inspired by the multi-phenotypic cellular environment of bone. Based on the deconstruction of the â stem cell nicheâ , the developed capsules are designed to drive a self-regulated osteogenesis. PLLA microparticles functionalized with collagen I, and a co-culture of adipose stem (ASCs) and endothelial (ECs) cells are immobilized in spherical liquified capsules. The capsules are coated with multilayers of poly(L-lysine), alginate, and chitosan nano-assembled through layer-by-layer. Capsules encapsulating ASCs alone or in a co-culture with ECs are cultured in endothelial medium with or without osteogenic differentiation factors. Results show that osteogenesis is enhanced by the co-encapsulation, which occurs even in the absence of differentiation factors. These findings are supported by an increased ALP activity and matrix mineralization, osteopontin detection, and the up regulation ofÂ BMP-2, RUNX2Â andÂ BSP. The liquified co-capsules also act as a VEGF and BMP-2 cytokines release system. The proposed liquified capsules might be a valuable injectable self-regulated system for bone regeneration employing highly translational cell sources.|
|Appears in Collections:||3B’s - Artigos em revistas/Papers in scientific journals|