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TitleMicroscopy- or DNA-based analyses: Which methodology gives a truer picture of stream-dwelling decomposer fungal diversity?
Author(s)Fernandes, Isabel Rodrigues
Pereira, Ana
Trabulo, José
Pascoal, Cláudia
Cássio, Fernanda
Duarte, Sofia Alexandra Ferreira
KeywordsAquatic hyphomycetes
454 pyrosequencing
Denaturing gradient gel electrophoresis
Fungal spores morphology
Issue date2015
JournalFungal Ecology
CitationFernandes, I., Pereira, A., Trabulo, J., Pascoal, C., Cássio, F., & Duarte, S. (2015). Microscopy- or DNA-based analyses: Which methodology gives a truer picture of stream-dwelling decomposer fungal diversity? Fungal Ecology. doi: 10.1016/j.funeco.2015.08.005
Abstract(s)We assessed aquatic hyphomycete diversity in autumn and spring on oak leaves decomposing in five streams along a gradient of eutrophication in the Northwest of Portugal. Diversity was assessed through microscopy-based (identification by spore morphology) and DNA-based techniques (Denaturing Gradient Gel Electrophoresis and 454 pyrosequencing). Pyrosequencing revealed five times greater diversity than DGGE. About 21% of all aquatic hyphomycete species were exclusively detected by pyrosequencing and 26% exclusively by spore identification. In some streams, more than half of the recorded species would have remained undetected if we had relied only on spore identification. Nevertheless, in spring aquatic hyphomycete diversity was higher based on spore identification, probably because many species occurring in this season are not yet connected to ITS barcodes in genetic databases. Pyrosequencing was a powerful tool for revealing aquatic hyphomycete diversity on decomposing plant litter in streams and we strongly encourage researchers to continue the effort in barcoding fungal species.
Publisher versionThe original publication is available at
AccessOpen access
Appears in Collections:DBio - Artigos/Papers

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