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TitleDevelopment of elastin-like recombinamer films with antimicrobial activity
Author(s)da Costa, André
Machado, Raul
Ribeiro, Artur
Collins, Tony
Thiagarajan, Viruthachalam
Neves-Petersen, Maria Teresa
Rodríguez-Cabello, José Carlos
Gomes, Andreia C.
Casal, Margarida
Anti-Infective Agents
Cell Line
Drug Carriers
Escherichia coli
Skin Absorption
Issue date9-Feb-2015
PublisherAmerican Chemical Society
Citationda Costa, A., Machado, R., Ribeiro, A., Collins, T., Thiagarajan, V., Neves-Petersen, M. T., . . . Casal, M. (2015). Development of Elastin-Like Recombinamer Films with Antimicrobial Activity. Biomacromolecules, 16(2), 625-635. doi: 10.1021/bm5016706
Abstract(s)In the present work we explored the ABP-CM4 peptide properties from Bombyx mori for the creation of biopolymers with broad antimicrobial activity. An antimicrobial recombinant protein-based polymer (rPBP) was designed by cloning the DNA sequence coding for ABP-CM4 in frame with the N-terminus of the elastin-like recombinamer consisting of 200 repetitions of the pentamer VPAVG, here named A200. The new rPBP, named CM4-A200, was purified via a simplified nonchromatographic method, making use of the thermoresponsive behavior of the A200 polymer. ABP-CM4 peptide was also purified through the incorporation of a formic acid cleavage site between the peptide and the A200 sequence. In soluble state the antimicrobial activity of both CM4-A200 polymer and ABP-CM4 peptide was poorly effective. However, when the CM4-A200 polymer was processed into free-standing films high antimicrobial activity against Gram-positive and Gram-negative bacteria, yeasts and filamentous fungi was observed. The antimicrobial activity of CM4-A200 was dependent on the physical contact of cells with the film surface. Furthermore, CM4-A200 films did not reveal a cytotoxic effect against both normal human skin fibroblasts and human keratinocytes. Finally, we have developed an optimized ex vivo assay with pig skin demonstrating the antimicrobial properties of the CM4-A200 cast films for skin applications.
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