Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/40673

TitleA novel hanging spherical drop system for the generation of cellular spheroids and high throughput combinatorial drug screening
Author(s)Neto, A. I.
Correia, C. R.
Oliveira, M. B.
Rial-Hermida, M. I.
Álvarez-Lorenzo, C.
Reis, R. L.
Mano, J. F.
KeywordsHigh-throughput screening
Spheroids
Issue dateJan-2015
PublisherRoyal Society of Chemistry
JournalBiomaterials Science
CitationNeto, A. I., Correia, C. R., Oliveira, M. B., Rial-Hermida, M. I., Alvarez-Lorenzo, C., Reis, R. L., & Mano, J. F. (2015). A novel hanging spherical drop system for the generation of cellular spheroids and high throughput combinatorial drug screening. Biomaterials Science, 3(4), 581-585. doi: 10.1039/c4bm00411f
Abstract(s)We propose a novel hanging spherical drop system for anchoring arrays of droplets of cell suspension based on the use of biomimetic superhydrophobic flat substrates, with controlled positional adhesion and minimum contact with a solid substrate. By facing down the platform, it was possible to generate independent spheroid bodies in a high throughput manner, in order to mimic in vivo tumour models on the lab-on-chip scale. To validate this system for drug screening purposes, the toxicity of the anti-cancer drug doxorubicin in cell spheroids was tested and compared to cells in 2D culture. The advantages presented by this platform, such as feasibility of the system and the ability to control the size uniformity of the spheroid, emphasize its potential to be used as a new low cost toolbox for high-throughput drug screening and in cell or tissue engineering.
TypeArticle
URIhttp://hdl.handle.net/1822/40673
DOI10.1039/C4BM00411F
ISSN2047-4830
Publisher versionhttp://dx.doi.org/10.1039/c4bm00411f
Peer-Reviewedyes
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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