Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/40591

TitlePDMS biofunctionalization study for the development of a microfluidic device: Application to salivary cortisol
Author(s)Pinto, Vânia Cristina Gonçalves
Minas, Graça
Neves, Margarida Correia
KeywordsPoly(dimethylsiloxane) (PDMS)
Microfluidic immunosensor
surface biofunctionalization
Issue date26-Feb-2015
PublisherIEEE
CitationPinto, V. C., Minas, G., & Correia-Neves, M. (2015). PDMS biofunctionalization study for the development of a microfluidic device: Application to salivary cortisol. Paper presented at the Proceedings - 2015 IEEE 4th Portuguese Meeting on Bioengineering, ENBENG 2015.
Abstract(s)This paper presents the study of the PDMS (poly(dimethylsiloxane)) surface functionalization for the development of a microfluidic immunosensor that quantitatively analyss salivary cortisol by optical detection. The functionalization was performed using different antibodies immobilization methods on PDMS surface: (a) immobilization by passive adsorption on pristine PDMS; (b) silanization of PDMS surface with (3-aminopropyl)-triethoxysilane (APTES) to generate amino groups and posterior covalent immobilization of antibodies on APTES-PDMS using cross-linker glutaraldehyde (GA); (c) coating the PDMS surface with BSA to block non-specific protein adsorption, and then covalent bond of the protein A via GA. In this last approach, the antibodies were covalently immobilized to protein A due to its high affinity with the constant fraction (Fc) region of the antibodies. Atomic force microscope (AFM) and spectrophotometric analysis demonstrated that the immobilization method using protein A is more efficient since a higher roughness and uniformity on the PDMS surface and higher absorbance signals were obtained
TypeConference paper
DescriptionThe authors acknowledge to Sofia Neves from ICVS for her help in the antibodies selection.
URIhttp://hdl.handle.net/1822/40591
ISBN9781479982691
DOI10.1109/ENBENG.2015.7088852
Publisher versionhttp://dx.doi.org/10.1109/ENBENG.2015.7088852
Peer-Reviewedyes
AccessRestricted access (UMinho)
Appears in Collections:DEI - Artigos em atas de congressos internacionais
ICVS - Artigos em Revistas Internacionais com Referee

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