Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/40591
Título: | PDMS biofunctionalization study for the development of a microfluidic device: Application to salivary cortisol |
Autor(es): | Pinto, Vânia Cristina Gonçalves Minas, Graça Neves, Margarida Correia |
Palavras-chave: | Poly(dimethylsiloxane) (PDMS) Microfluidic immunosensor surface biofunctionalization |
Data: | 26-Fev-2015 |
Editora: | IEEE |
Citação: | Pinto, V. C., Minas, G., & Correia-Neves, M. (2015). PDMS biofunctionalization study for the development of a microfluidic device: Application to salivary cortisol. Paper presented at the Proceedings - 2015 IEEE 4th Portuguese Meeting on Bioengineering, ENBENG 2015. |
Resumo(s): | This paper presents the study of the PDMS (poly(dimethylsiloxane)) surface functionalization for the development of a microfluidic immunosensor that quantitatively analyss salivary cortisol by optical detection. The functionalization was performed using different antibodies immobilization methods on PDMS surface: (a) immobilization by passive adsorption on pristine PDMS; (b) silanization of PDMS surface with (3-aminopropyl)-triethoxysilane (APTES) to generate amino groups and posterior covalent immobilization of antibodies on APTES-PDMS using cross-linker glutaraldehyde (GA); (c) coating the PDMS surface with BSA to block non-specific protein adsorption, and then covalent bond of the protein A via GA. In this last approach, the antibodies were covalently immobilized to protein A due to its high affinity with the constant fraction (Fc) region of the antibodies. Atomic force microscope (AFM) and spectrophotometric analysis demonstrated that the immobilization method using protein A is more efficient since a higher roughness and uniformity on the PDMS surface and higher absorbance signals were obtained |
Tipo: | Artigo em ata de conferência |
Descrição: | The authors acknowledge to Sofia Neves from ICVS for her help in the antibodies selection. |
URI: | https://hdl.handle.net/1822/40591 |
ISBN: | 9781479982691 |
DOI: | 10.1109/ENBENG.2015.7088852 |
Versão da editora: | http://dx.doi.org/10.1109/ENBENG.2015.7088852 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | DEI - Artigos em atas de congressos internacionais |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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BioEng2015_IEEE_07088852_Vania.pdf Acesso restrito! | 817,06 kB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons