Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/40317

TitleRegulation of human mesenchymal stem cell osteogenesis by specific surface density of fibronectin: a gradient study
Author(s)Torres, Ana B. Faia
Goren, T.
Ihalainen, T. O.
Guimond-Lischer, S.
Charnley, M.
Rottmar, M.
Maniura-Weber, K.
Spencer, N. D.
Reis, R. L.
Textor, M.
Neves, N. M.
KeywordsBiomaterials
Fibronectin
Gradient
Mesenchymal stem cells
Osteogenesis
Issue date2015
PublisherACS Publications
JournalACS Applied Materials and Interfaces
Citation44. Faia-Torres, A. B., Goren, T., Ihalainen, T. O., Guimond-Lischer, S., Charnley, M., Rottmar, M., . . . Neves, N. M. (2015). Regulation of Human Mesenchymal Stem Cell Osteogenesis by Specific Surface Density of Fibronectin: a Gradient Study. Acs Applied Materials & Interfaces, 7(4), 2367-2375. doi: 10.1021/am506951c
Abstract(s) The success of synthetic bone implants requires good interface between the material and the host tissue. To study the biological relevance of fi bronectin (FN) density on the osteogenic commitment of human bone marrow mesenchymal stem cells (hBMMSCs), human FN was adsorbed in a linear density gradient on the surface of PCL. The evolution of the osteogenic markers alkaline phosphatase and collagen 1 alpha 1 was monitored by immunohistochemistry, and the cytoskeletal organization and the cell-derived FN were assessed. The functional analysis of the gradient revealed that the lower FN-density elicited stronger osteogenic expression and higher cytoskeleton spreading, hallmarks of the stem cell commitment to the osteoblastic lineage. The identifi cation of the optimal FN density regime for the osteogenic commitment of hBM-MSCs presents a simple and versatile strategy to signifi cantly enhance the surface properties of polycaprolactone as a paradigm for other synthetic polymers intended for bone-related applications.
TypeArticle
URIhttp://hdl.handle.net/1822/40317
DOI10.1021/am506951c
ISSN1944-8252
1944-8244
Publisher versionhttp://pubs.acs.org/doi/abs/10.1021/am506951c
Peer-Reviewedyes
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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