Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/40309

TitleAcetylated bacterial cellulose coated with urinary bladder matrix as a substrate for retinal pigment epithelium
Author(s)Gonçalves, S.
Rodrigues, I. P.
Padrão, Jorge
Silva, João P.
Sencadas, Vítor João Gomes Silva
Lanceros-Méndez, S.
Girão, H.
Gama, F. M.
Dourado, Fernando
Rodrigues, L. R.
KeywordsAcetylated bacterial cellulose
Urinary bladder matrix
Retinal degenerative diseases
Retinal pigment epithelium
Issue dateMar-2016
PublisherElsevier B.V.
JournalColloids and Surfaces B: Biointerfaces
CitationGonçalves, S.; Rodrigues, I. P.; Padrão, Jorge; Silva, João P.; Sencadas, V.; Lanceros-Mendez, S.; Girão, H.; Gama, F. M.; Dourado, Fernando; Rodrigues, L. R., Acetylated bacterial cellulose coated with urinary bladder matrix as a substrate for retinal pigment epithelium. Colloids and Surfaces B: Biointerfaces, 139, 1-9, 2016
Abstract(s)This work evaluated the effect of acetylated bacterial cellulose (ABC) substrates coated with urinary bladder matrix (UBM) on the behavior of Retinal Pigment Epithelium (RPE), as assessed by cell adhesion, proliferation and development of cell polarity exhibiting transepithelial resistance and polygonal shaped-cells with microvilli. Acetylation of bacterial cellulose (BC) generated a moderate hydrophobic surface (around 65°) while the adsorption of UBM onto these acetylated substrates did not affect significantly the surface hydrophobicity. The ABS substrates coated with UBM enabled the development of a cell phenotype closer to that of native RPE cells. These cells were able to express proteins essential for their cytoskeletal organization and metabolic function (ZO-1 and RPE65), while showing a polygonal shaped morphology with microvilli and a monolayer configuration. The coated ABC substrates were also characterized, exhibiting low swelling effect (between 1.52.0 swelling/mm3), high mechanical strength (2048 MPa) and non-pyrogenicity (2.12 EU/L). Therefore, the ABC substrates coated with UBM exhibit interesting features as potential cell carriers in RPE transplantation that ought to be further explored.
TypeArticle
URIhttp://hdl.handle.net/1822/40309
DOI10.1016/j.colsurfb.2015.11.051
ISSN0927-7765
e-ISSN0927-7765
Publisher versionhttp://www.journals.elsevier.com/colloids-and-surfaces-b-biointerfaces/
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series
CDF - FCD - Artigos/Papers (with refereeing)

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