Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/40302

TítuloPlatelet lysate-based pro-angiogenic nanocoatings
Autor(es)Oliveira, Sara M.
Pirraco, Rogério P.
Marques, A. P.
Santo, Vítor E.
Gomes, Manuela E.
Reis, R. L.
Mano, J. F.
Palavras-chaveLayer-by-layer assembling
Instructive surfaces
Platelet lysate
Growth factors
Angiogenesis
Endothelial cells
DataDez-2015
EditoraElsevier
RevistaActa Biomaterialia
CitaçãoOliveira S. M., Pirraco R. P., Marques A. P., Santo V. E., Gomes M. E., Reis R. L., Mano J. F. Platelet lysate-based pro-angiogenic nanocoatings, Acta Biomaterialia, pp. in press, doi:10.1016/j.actbio.2015.12.028, 2015
Resumo(s)Human platelet lysate (PL) is a cost-effective and human source of autologous multiple and potent pro-angiogenic factors, such as vascular endothelial growth factor A (VEGF A), fibroblast growth factor b (FGF b) and angiopoietin-1. Nanocoatings previously characterized were prepared by layer-by-layer assembling incorporating PL with marine-origin polysaccharides and were shown to activate human umbilical vein endothelial cells (HUVECs). Within 20 h of incubation, the more sulfated coatings induced the HUVECS to the form tube-like structures accompanied by an increased expression of angiogenicassociated genes, such as angiopoietin-1 and VEGF A. This may be a cost-effective approach to modify 2D/3D constructs to instruct angiogenic cells towards the formation of neo-vascularization, driven by multiple and synergistic stimulations from the PL combined with sulfated polysaccharides. Statement of Significance The presence, or fast induction, of a stable and mature vasculature inside 3D constructs is crucial for new tissue formation and its viability. This has been one of the major tissue engineering challenges, limiting the dimensions of efficient tissue constructs. Many approaches based on cells, growth factors, 3D bioprinting and channel incorporation have been proposed. Herein, we explored a versatile technique, layer-by-layer assembling in combination with platelet lysate (PL), that is a cost-effective source of many potent pro-angiogenic proteins and growth factors. Results suggest that the combination of PL with sulfated polyelectrolytes might be used to introduce interfaces onto 2D/3D constructs with potential to induce the formation of cell-based tubular structures.
Tipoarticle
URIhttp://hdl.handle.net/1822/40302
DOI10.1016/j.actbio.2015.12.028
ISSN1742-7061
Versão da editorahttp://www.sciencedirect.com/science/article/pii/S1742706115302658
Arbitragem científicayes
AcessoopenAccess
Aparece nas coleções:3B’s - Artigos em revistas/Papers in scientific journals

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