Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/40301

Full metadata record
DC FieldValueLanguage
dc.contributor.authorBoas, Diana Patrícia Andrade Vilaspor
dc.contributor.authorAlmeida, Carinapor
dc.contributor.authorSillankorva, Sannapor
dc.contributor.authorNicolau, Anapor
dc.contributor.authorAzeredo, Joanapor
dc.contributor.authorAzevedo, Nuno F.por
dc.date.accessioned2016-02-15T14:57:28Z-
dc.date.available2016-02-15T14:57:28Z-
dc.date.issued2016-
dc.identifier.citationVilas Boas, Diana; Almeida, Carina; Sillankorva, Sanna; Nicolau, Ana; Azeredo, Joana; Azevedo, Nuno F., Discrimination of bacteriophage infected cells using locked nucleic acid fluorescent in situ hybridization (LNA-FISH). Biofouling, 32(2), 179-190, 2016por
dc.identifier.issn0892-7014por
dc.identifier.issn1029-2454por
dc.identifier.urihttp://hdl.handle.net/1822/40301-
dc.description.abstractBacteriophage-host interaction studies in biofilm structures are still challenging due to the technical limitations of traditional methods. The aim of this study was to provide a direct fluorescence in situ hybridization (FISH) method based on locked nucleic acid (LNA) probes, which targets the phage replication phase, allowing the study of population dynamics during infection. Bacteriophages specific for two biofilm-forming bacteria, Pseudomonas aeruginosa and Acinetobacter, were selected. Four LNA probes were designed and optimized for phage-specific detection and for bacterial counterstaining. To validate the method, LNA-FISH counts were compared with the traditional plaque forming unit (PFU) technique. To visualize the progression of phage infection within a biofilm, colony-biofilms were formed and infected with bacteriophages. A good correlation (r=0.707) was observed between LNA-FISH and PFU techniques. In biofilm structures, LNA-FISH provided a good discrimination of the infected cells and also allowed the assessment of the spatial distribution of infected and non-infected populations.por
dc.description.sponsorshipThis work was supported by the Portuguese Science Foundation [project RECI/BBB-EBI/0179/2012 (FCOMP-01-0124- FEDER-027462)], [project IF/01413/2013]; and DNA mimics [ref. PIC/IC/82815/ 2007] from Fundação para a Ciência e Tecnologia (FCT) and Ministério da Ciência, Tecnologia e Ensino Superior (MCTES).por
dc.language.isoengpor
dc.publisherTaylor & Francispor
dc.relationinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/126270/PTpor
dc.relationFCOMP-01-0124- FEDER-027462por
dc.relationIF/01413/2013por
dc.relationPIC/IC/82815/ 2007por
dc.rightsopenAccesspor
dc.subjectPseudomonas aeruginosapor
dc.subjectAcinetobacterpor
dc.subjectBacteriophagepor
dc.subjectBiofilmspor
dc.subjectPhage infectionpor
dc.subjectLNA-FISHpor
dc.subjectphage infection, LNA-FISHpor
dc.titleDiscrimination of bacteriophage infected cells using locked nucleic acid fluorescent in situ hybridization (LNA-FISH)por
dc.typearticle-
dc.peerreviewedyespor
dc.commentsCEB33788por
sdum.publicationstatuspublishedpor
oaire.citationStartPage179por
oaire.citationEndPage190por
oaire.citationIssue2por
oaire.citationTitleBiofoulingpor
oaire.citationVolume32por
dc.date.updated2016-02-03T22:11:53Z-
dc.identifier.doi10.1080/08927014.2015.1131821por
dc.identifier.pmid26813295por
dc.subject.wosScience & Technologypor
sdum.journalBiofoulingpor
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

Files in This Item:
File Description SizeFormat 
document_33788_1.pdf1,96 MBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID