Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/40286

TitleOsteogenic differentiation of human mesenchymal stem cells in the absence of osteogenic supplements: a surface-roughness gradient study
Author(s)Torres, Ana B. Faia
Charnley, Mirren
Goren, Tolga
Lischer, Stefanie Guimond
Rottmar, Markus
Weber, Katharina Maniura
Spencer, Nicholas D.
Reis, R. L.
Textor, Marcus
Neves, N. M.
KeywordsBiomaterials
High-throughput screening (gradient)
Microstructures
Mesenchymal stem cells
Osteogenesis
Issue dateOct-2015
PublisherElsevier
JournalActa Biomaterialia
CitationFaia-Torres A. B., Charnley M., Goren T., Guimond-Lischer S., Rottmar M., Maniura-Weber K., Spencer N. D., Reis R. L., Textor M., Neves N. M. Osteogenic differentiation of human mesenchymal stem cells in the absence of osteogenic supplements: a surface-roughness gradient study., Acta Biomaterialia, Vol. 28, pp. 64-75, doi:10.1016/j.actbio.2015.09.028, 2015
Abstract(s)The use of biomaterials to direct osteogenic differentiation of human mesenchymal stem cells (hMSCs) in the absence of osteogenic supplements is thought to be part of the next generation of orthopedic implants. We previously engineered surface-roughness gradients of average roughness (Ra) varying from the sub-micron to the micrometer range ( 0.5–4.7 lm), and mean distance between peaks (RSm) gradually varying from 214 lm to 33 lm. Here we have screened the ability of such surface-gradients of polycaprolactone to influence the expression of alkaline phosphatase (ALP), collagen type 1 (COL1) and mineralization by hMSCs cultured in dexamethasone (Dex)-deprived osteogenic induction medium (OIM) and in basal growth medium (BGM). Ra 1.53 lm/RSm 79 lm in Dex-deprived OI medium, and Ra 0.93 lm/RSm 135 lm in BGM consistently showed higher effectiveness at supporting the expression of the osteogenic markers ALP, COL1 and mineralization, compared to the tissue culture polystyrene (TCP) control in complete OIM. The superior effectiveness of specific surface-roughness revealed that this strategy may be used as a compelling alternative to soluble osteogenic inducers in orthopedic applications featuring the clinically relevant biodegradable polymer polycaprolactone.
TypeArticle
URIhttp://hdl.handle.net/1822/40286
DOI10.1016/j.actbio.2015.09.028
ISSN1742-7061
Publisher versionhttp://www.sciencedirect.com/science/article/pii/S1742706115301215
Peer-Reviewedyes
AccessRestricted access (UMinho)
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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