Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/40286

TítuloOsteogenic differentiation of human mesenchymal stem cells in the absence of osteogenic supplements: a surface-roughness gradient study
AutorTorres, Ana B. Faia
Charnley, Mirren
Goren, Tolga
Lischer, Stefanie Guimond
Rottmar, Markus
Weber, Katharina Maniura
Spencer, Nicholas D.
Reis, R. L.
Textor, Marcus
Neves, N. M.
Palavras-chaveBiomaterials
High-throughput screening (gradient)
Microstructures
Mesenchymal stem cells
Osteogenesis
DataOut-2015
EditoraElsevier
CitaçãoFaia-Torres A. B., Charnley M., Goren T., Guimond-Lischer S., Rottmar M., Maniura-Weber K., Spencer N. D., Reis R. L., Textor M., Neves N. M. Osteogenic differentiation of human mesenchymal stem cells in the absence of osteogenic supplements: a surface-roughness gradient study., Acta Biomaterialia, Vol. 28, pp. 64-75, doi:10.1016/j.actbio.2015.09.028, 2015
ResumoThe use of biomaterials to direct osteogenic differentiation of human mesenchymal stem cells (hMSCs) in the absence of osteogenic supplements is thought to be part of the next generation of orthopedic implants. We previously engineered surface-roughness gradients of average roughness (Ra) varying from the sub-micron to the micrometer range ( 0.5–4.7 lm), and mean distance between peaks (RSm) gradually varying from 214 lm to 33 lm. Here we have screened the ability of such surface-gradients of polycaprolactone to influence the expression of alkaline phosphatase (ALP), collagen type 1 (COL1) and mineralization by hMSCs cultured in dexamethasone (Dex)-deprived osteogenic induction medium (OIM) and in basal growth medium (BGM). Ra 1.53 lm/RSm 79 lm in Dex-deprived OI medium, and Ra 0.93 lm/RSm 135 lm in BGM consistently showed higher effectiveness at supporting the expression of the osteogenic markers ALP, COL1 and mineralization, compared to the tissue culture polystyrene (TCP) control in complete OIM. The superior effectiveness of specific surface-roughness revealed that this strategy may be used as a compelling alternative to soluble osteogenic inducers in orthopedic applications featuring the clinically relevant biodegradable polymer polycaprolactone.
Tipoarticle
URIhttp://hdl.handle.net/1822/40286
DOI10.1016/j.actbio.2015.09.028
ISSN1742-7061
Versão da editorahttp://www.sciencedirect.com/science/article/pii/S1742706115301215
Arbitragem científicayes
AcessorestrictedAccess
Aparece nas coleções:3B’s - Artigos em revistas/Papers in scientific journals

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