Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/40192

TitleEnzymatic phosphorylation of hair keratin enhances fast adsorption of cationic moieties
Author(s)Volkov, Vadim
Cavaco-Paulo, Artur
KeywordsHair keratin
Methylene blue
Phosphorylation
Issue dateApr-2016
PublisherElsevier B.V.
JournalInternational Journal of Biological Macromolecules
CitationVolkov, V.; Paulo, Artur Cavaco, Enzymatic phosphorylation of hair keratin enhances fast adsorption of cationic moieties. International Journal of Biological Macromolecules, 85, 476-486, 2016
Abstract(s)The current study describes the in vitro phosphorylation of a human hair keratin, using protein kinase for the first time. Phosphorylation of keratin was demonstrated by 31P NMR (Nuclear Magnetic Resonance) and Diffuse Reflectance Infrared Fourier Transform (DRIFT) techniques. Phosphorylation induced a 2.5 fold increase of adsorption capacity in the first 10 minutes for cationic moiety like Methylene Blue (MB). Thorough description of MB adsorption process was performed by several isothermal models. Reconstructed fluorescent microscopy images depict distinct amounts of dye bound to the differently treated hair. The results of this work suggest that the enzymatic phosphorylation of keratins might have significant implications in hair shampooing and conditioning, where short application times of cationic components are of prime importance.
TypeArticle
URIhttp://hdl.handle.net/1822/40192
DOI10.1016/j.ijbiomac.2015.12.082
ISSN0141-8130
Publisher versionhttp://www.elsevier.com/locate/issn/01418130
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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