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TitleAn efficient chronic unpredictable stress protocol to induce stress-relatec responses in C57BL/6 mice
Author(s)Monteiro, S.
Roque, Susana
Calçada, Daniela Alexandra Sá
Sousa, Nuno
Neves, Margarida Correia
Cerqueira, João José
KeywordsChronic stress
Neuropsychiatric disorders
Immune dysfunction
Depressive-like behavior
Social defeat
Issue date2015
PublisherFrontiers Media
JournalFrontiers in Psychiatry
CitationMonteiro, S., Roque, S., de Sá-Calçada, D., Sousa, N., Correia-Neves, M., & Cerqueira, J. J. (2015). An efficient chronic unpredictable stress protocol to induce stress-related responses in C57BL/6 mice. Frontiers in Psychiatry, 6(FEB). doi: 10.3389/fpsyt.2015.00006
Abstract(s)Exposure to chronic stress can have broad effects on health ranging from increased predisposition for neuropsychiatric disorders to deregulation of immune responses. The chronic unpredictable stress (CUS) protocol has been widely used to study the impact of stress exposure in several animal models and consists in the random, intermittent, and unpredictable exposure to a variety of stressors during several weeks. CUS has consistently been shown to induce behavioral and immunological alterations typical of the chronic stress-response. Unfortunately C57BL/6 mice, one of the most widely used mouse strains, due to the great variety of genetically modified lines, seem to be resistant to the commonly used 4-week-long CUS protocol. The definition of an alternative CUS protocol allowing the use of C57BL/6 mice in chronic stress experiments is a need. Here, we show that by extending the CUS protocol to 8?weeks is possible to induce a chronic stress-response in C57BL/6 mice, as revealed by abrogated body weight gain, increased adrenals weight, and an overactive hypothalamic-pituitary-adrenal axis with increased levels of serum corticosterone. Moreover, we also observed stress-associated behavioral alterations, including the potentiation of anxious-like and depressive-like behaviors and a reduction of exploratory behavior, as well as subtle stress-related changes in the cell population of the thymus and of the spleen. The present protocol for C57BL/6 mice consistently triggers the spectrum of CUS-induced changes observed in rats and, thus, will be highly useful to researchers that need to use this particular mouse strain as an animal model of neuropsychiatric disorders and/or immune deregulation related to CUS.
Publisher version
AccessOpen access
Appears in Collections:ICVS - Artigos em Revistas Internacionais com Referee

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