Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/39023

TitleModification of microfiltration membranes by hydrogel impregnation for pDNA purification
Author(s)Castilho, Pedro H.
Correia, Tiago R.
Amorim, M. T. Pessoa de
Escobar, Isabel C.
Queiroz, João A.
Correia, Ilídio J.
Morão, António M.
KeywordsMembranes
Nucleic acids
Polysaccharides
Issue date2015
PublisherWiley
JournalJournal of Applied Polymer Science
CitationCastilho, P. H., Correia, T. R., Pessoa De Amorim, M. T., Escobar, I. C., Queiroz, J. A., Correia, I. J., & Morão, A. M. (2015). Modification of microfiltration membranes by hydrogel impregnation for pDNA purification. Journal of Applied Polymer Science, 132(21). doi: 10.1002/app.41610
Abstract(s)The huge efforts for the achievement of highly purified biomolecules are growing every day. A great number of efficient techniques, such as chromatography, are already available in laboratory for separation processes. However, membrane-based technologies are the best match to assure simplicity, efficiency and easy scale-up procedures. Herein we report the modification of a commercial microfiltration membrane for plasmid DNA purification by agarose gel impregnation. The membrane was characterized by SEM, ATR-FTIR, EDS, contact angle, and porosity measurements. Additionally, the membrane pore radius was estimated from observed rejections of different proteins and with that information the rejection of a 6050 bp plasmid DNA (pDNA) molecule was estimated for different values of flux using a theoretical model of large flexible molecules in membranes with parallel cylindrical pores, which is applicable to pDNA ultrafiltration in conventional membranes, as recently shown in the literature. The experimental results show that the modified membrane has higher pDNA rejections than the predicted by the model, suggesting that the different type of porous structure that a hydrogel has, may have a positive effect on pDNA rejections as compared to other biomolecules with more rigid structures, making this type of modified membranes potential better candidates to be used for the selective recovery of pDNA in this type of bioprocesses.
TypeArticle
URIhttp://hdl.handle.net/1822/39023
DOI10.1002/app.41610
ISSN0021-8995
Peer-Reviewedyes
AccessOpen access
Appears in Collections:DET/2C2T - Artigos em revistas internacionais com arbitragem científica

Files in This Item:
File Description SizeFormat 
Castilho_et_al-2015-Journal_of_Applied_Polymer_Science (1).pdf1,36 MBAdobe PDFView/Open

This item is licensed under a Creative Commons License Creative Commons

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID