Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/38828

TitleMechanical fatigue performance of PCL-chondroprogenitor constructs after cell culture under bioreactor mechanical stimulus
Author(s)Panadero, J. A.
Sencadas, Vítor João Gomes Silva
Silva, S. C. M.
Ribeiro, C.
Correia, V.
Gama, F. M.
Gomez Ribelles, J. L.
Lanceros-Méndez, S.
KeywordsPoly-ε-caprolactone
Fatigue testing
Bioreactor
Biomaterials
Fibrin
poly-É-caprolactone
Issue date2016
PublisherWiley
JournalJournal of Biomedical Materials Research Part B: Applied Biomaterials
CitationPanadero, J. A., Sencadas, V., Silva, S. C. M., Ribeiro, C., Correia, V., Gama, F. M., . . . Lanceros-Mendez, S. (2016). Mechanical fatigue performance of PCL-chondroprogenitor constructs after cell culture under bioreactor mechanical stimulus. Journal of Biomedical Materials Research - Part B Applied Biomaterials. doi: 10.1002/jbm.b.33386
Abstract(s)In tissue engineering of cartilage, polymeric scaffolds are implanted in the damaged tissue and subjected to repeated compression loading cycles. The possibility of failure due to mechanical fatigue has not been properly addressed in these scaffolds. Nevertheless, the macroporous scaffold is susceptible to failure after repeated loading-unloading cycles. This is related to inherent discontinuities in the material due to the micropore structure of the macro-pore walls that act as stress concentration points. In this work, chondrogenic precursor cells have been seeded in Poly-ε-caprolactone (PCL) scaffolds with fibrin and some were submitted to free swelling culture and others to cyclic loading in a bioreactor. After cell culture, all the samples were analyzed for fatigue behavior under repeated loading-unloading cycles. Moreover, some components of the extracellular matrix (ECM) were identified. No differences were observed between samples undergoing free swelling or bioreactor loading conditions, neither respect to matrix components nor to mechanical performance to fatigue. The ECM did not achieve the desired preponderance of collagen type II over collagen type I which is considered the main characteristic of hyaline cartilage ECM. However, prediction in PCL with ECM constructs was possible up to 600 cycles, an enhanced performance when compared to previous works. PCL after cell culture presents an improved fatigue resistance, despite the fact that the measured elastic modulus at the first cycle was similar to PCL with poly(vinyl alcohol) samples. This finding suggests that fatigue analysis in tissue engineering constructs can provide additional information missed with traditional mechanical measurements.
TypeArticle
URIhttp://hdl.handle.net/1822/38828
DOI10.1002/jbm.b.33386
ISSN1552-4973
1552-4981
Peer-Reviewedyes
AccessRestricted access (UMinho)
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series
CDF - FCD - Artigos/Papers (with refereeing)

Files in This Item:
File Description SizeFormat 
Panadero_2015.pdf
  Restricted access
830,82 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID