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|Title:||Tendon regeneration through a scaffold-free approach: development of tenogenic magnetic hASCs sheets|
|Author(s):||Gonçalves, A. I.|
Rodrigues, M. T.
Reis, R. L.
Gomes, Manuela E.
|Publisher:||Mary Ann Liebert|
|Citation:||Gonçalves A. I., Rodrigues M. T., Reis R. L., Gomes M. E. Tendon Regeneration Through a Scaffold-free Approach: Development of Tenogenic Magnetic hASCs Sheets, TISSUE ENGINEERING: Part A, Vol. 21, Issue S1, pp. S-357, doi:10.1089/ten.tea.2015.5000.abstracts, 2015|
|Abstract(s):||Tendon's regeneration is limited, demanding for cell-based strategies to fully restore their functionality upon injury. The concept of magnetic force-based TE(1), generally using magnetic nanoparticles may enable, for example, stem cell stimulation and/or remote control over TE constructs. Thus, we originally propose the development of magnetic cell sheets (magCSs) with tenogenic capability, aimed at promoting tendon's regeneration. A Tenomodulin (TNMD+) subpopulation was sorted from human adipose stem cells (hASCs), using TNMD-coated immunomagnetic beads(2) and used as cell source for the development of magCSs. Briefly, cells were labeled with iron oxide composite particles (Micromod) and cultured for 7 days in Î±-MEM medium with or without magnetic stimulation provided by a magnetic device (nanoTherics). CSs were retrieved from the plates using magnet attraction as contiguous sheets of cells within its own deposited ECM.|
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