Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/38477

TítuloStromal vascular fraction cell sheets angiogenic potential for tissue engineering and regenerative medicine applications
Autor(es)Costa, M.
Cerqueira, M. T.
Rodrigues, D. B.
Santos, T. C.
Marques, A. P.
Pirraco, Rogério P.
Reis, R. L.
Palavras-chaveIn vivo
Regenerative medicine
Tissue engineering
EditoraMary Ann Liebert
RevistaTissue Engineering. Part A
CitaçãoCosta M., Cerqueira M. T., Rodrigues D. B., Santos T. C., Marques A. P., Pirraco R. P., Reis R. L. Stromal Vascular Fraction Cell Sheets Angiogenic Potential for Tissue Engineering and Regenerative Medicine Applications, Tissue Engineering Part A, Vol. 21, Issue S1, doi:10.1089/ten.tea.2015.5000.abstracts., 2015
Resumo(s)One of the biggest concerns in the Tissue Engineering field is the correct vascularization of engineered constructs. Strategies involving the use of endothelial cells are promising but adequate cell sourcing and neo-vessels stability are enduring challenges. In this work, we propose the hypoxic pre-conditioning of the stromal vascular fraction (SVF) of human adipose tissue to obtain highly angiogenic cell sheets (CS). For that, SVF was isolated after enzymatic dissociation of adipose tissue and cultured until CS formation in normoxic (pO2=21%) and hypoxic (pO2=5%) conditions for 5 and 8 days, in basal medium. Immunocytochemistry against CD31 and CD146 revealed the presence of highly branched capillary-like structures, which were far more complex for hypoxia. ELISA quantification showed increased VEGF and TIMP-1 secretion in hypoxia for 8 days of culture. In a Matrigel assay, the formation of capillary-like structures by endothelial cells was more prominent when cultured in conditioned medium recovered from the cultures in hypoxia. The same conditioned medium increased the migration of adipose stromal cells in a scratch assay, when compared with the medium from normoxia. Histological analysis after implantation of 8 days normoxic- and hypoxic-conditioned SVF CS in a hindlimb ischemia murine model showed improved formation of neo-blood vessels. Furthermore, Laser Doppler results demonstrated that the blood perfusion of the injured limb after 30 days was enhanced for the hypoxic CS group. Overall, these results suggest that SVF CS created under hypoxia can be used as functional vascularization units for tissue engineering and regenerative medicine.
Versão da editorahttp://online.liebertpub.com/doi/full/10.1089/ten.tea.2015.5000.abstracts
Arbitragem científicayes
Aparece nas coleções:3B’s - Resumos em livros de atas de conferências - indexados no ISI Web of Science

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