Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/38456

TítuloA continuous solvent- and oil-free method to prepare injectable PEGylated fibrinogen cell-laden microparticles
Autor(es)Oliveira, Mariana B.
Kossover, Olga
Mano, J. F.
Seliktar, Dror
EditoraMary Ann Liebert
RevistaTissue Engineering. Part A
CitaçãoOliveira M. B., Kossover O., Mano J. F., Seliktar D. A Continuous Solvent- and Oil-free Method to Prepare Injectable Pegylated Fibrinogen Cell-laden Microparticles, Tissue Engineering Part A, Vol. 21, Issue S1, pp. S1-S413, doi:10.1089/ten.tea.2015.5000.abstracts, 2015
Resumo(s)Injectable biomaterials with in situ cross-linking reactions have been suggested to minimize the invasiveness associated with most implantation procedures. However, problems related with the rapid liquid-to-gel transition reaction can arise because it is difficult to predict the reliability of the reaction and its end products, as well as to mitigate cytotoxicity to the surrounding tissues. An alternative minimally invasive approach to deliver solid implants in vivo is based on injectable microparticles, which can be processed in vitro with high fidelity and reliability, while showing low cytotoxicity. Their delivery to the defect can be performed by injection through a small diameter syringe needle. We present a new methodology for the continuous, solvent- and oil-free production of photopolymerizable microparticles containing encapsulated human dermal fibroblasts. A precursor solution of cells in photo-reactive PEG-fibrinogen (PF) polymer was transported through a transparent injector exposed to light-irradiation before being atomized in a jet-in-air nozzle. Shear rheometry data provided the cross-linking kinetics of each PF/cell solution, which was then used to determine the amount of irradiation required to partially polymerize the mixture prior to atomization. The partially polymerized drops fell into a gelation bath for further polymerization. The system was capable of producing cell-laden microparticles with high cellular viability, with an average diameter of between 88.1 µm to 347.1 µm and a dispersity of between 1.1 and 2.4, depending on the parameters chosen.
Versão da editorahttp://online.liebertpub.com/doi/full/10.1089/ten.tea.2015.5000.abstracts
Arbitragem científicayes
Aparece nas coleções:3B’s - Resumos em livros de atas de conferências - indexados no ISI Web of Science

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