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|Title:||Artificial thymus: a tissue engineering strategy|
|Author(s):||Costa-Pinto, A. R.|
Rodrigues, Bruno Cerqueira
Alves da Silva, Marta L.
Neves, N. M.
|Publisher:||Mary Ann Liebert|
|Journal:||Tissue Engineering. Part A|
|Citation:||Costa-Pinto A. R., Serre-Miranda C., Cerqueira-Rodrigues B., Martins A., Alves da Silva M. L., Correia-Neves M., Neves N. M. Artificial thymus_ a tissue engineering strategy, Tissue Engineering Part A, Vol. 21, pp. S-1-S-413, doi:10.1089/ten.tea.2015.5000.abstracts., 2015|
|Abstract(s):||The thymus is the central organ responsible for the generation of T lymphocytes (1). Various diseases cause the thymus to produce in- sufficient T cells, which can lead to immune-suppression (2). Since T cells are essential for the protection against pathogens, it is crucial to promote de novo differentiation of T cells on diseased individuals. The available clinical solutions are: 1) one protocol involving the transplant of thymic stroma from unrelated children only applicable for athymic children (3); 2) for patients with severe peripheral T cell depletion and reduced thymic activity, the administration of stimu- lating molecules stimulating the activity of the endogenous thymus (4). A scaffold (CellFoam) was suggested to support thymus regen- eration in vivo (5), although this research was discontinued. Herein, we propose an innovative strategy to generate a bioartificial thymus. We use a polycaprolactone nanofiber mesh (PCL-NFM) seeded and cultured with human thymic epithelial cells (hTECs). The cells were obtained from infant thymus collected during pediatric cardio-tho- racic surgeries. We report new data on the isolation and characterization of those cells and their interaction with PCL-NFM, by expanding hTECs into relevant numbers and by optimizing cell seeding methods.|
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