Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/37744

TitleExtracellular matrix in Staphylococcus epidermidis biofilms: a consequence of bacterial production or cell wall degradation?
Author(s)Cerca, Filipe
Andrade, Filipa
Correia, Alexandra
Teixeira, Luzia
Magalhães, Ângela
Hinzmann, Mariana
Ferreira, Íris
Ribeiro, Adília
Lobo-da-Cunha, Alexandre
Sampaio, Paula
Cerca, Nuno
Azeredo, Joana
Vilanova, Manuel
Issue dateSep-2009
CitationCerca, F.; Andrade, F.; Cerca, Nuno; Lobo-da-Cunha, A.; Azeredo, Joana; Vilanova, M., Bacterial population heterogeneity and extracellular matrix production in Staphylococcus epidermidis biofilms. EUROBIOFILMS 2009 - Book of Abstracts of the 1st European Congress on Microbial Biofilms. No. P184, Rome, Italy, September 2-5, 170-171, 2009.
Abstract(s)Staphylococcus epidermidis is a leading pathogen accounting for nosocomial infections. The ability to form biofilms is considered the major virulence factor of this bacterium. The hallmark of this type of infection is the presence of an extracellular polymeric matrix that, in the case of S. epidermidis biofilms, is mainly constituted by an N-acetylglucosamine polymer. We have identified a subpopulation of bacteria that we believe to be the responsible for the extracellular matrix accumulation in S. epidermidis biofilms as they have comparative significant higher amount of surface N-acetylglucosamine. Flow cytometric evaluation of cell wall permeability and transmission electronic microscopy are highly suggestive of primary wall degradation in these bacteria. In overall, these results suggest that the extracellular matrix in S. epidermidis biofilms is a consequence of the degradation of the bacteria cell wall and that propide iodium should be used with care when used as a marker for bacteria dead in biofilms.
TypeAbstract
URIhttp://hdl.handle.net/1822/37744
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Resumos em Livros de Atas / Abstracts in Proceedings

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