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TitleDevelopment of low-cost culture media for effective biosurfactant production
Author(s)Rodrigues, A. I.
Gudiña, Eduardo J.
Teixeira, J. A.
Rodrigues, L. R.
Issue dateSep-2015
CitationRodrigues, A.; Gudiña, Eduardo J.; Teixeira, J. A.; Rodrigues, L. R., Development of low-cost culture media for effective biosurfactant production. SINAFERM 2015 XX Simpósio Nacional de Bioprocessos. Fortaleza, Brazil, Sep. 1-4, 1-5, 2015.
Abstract(s)In this work, biosurfactant production by Pseudomonas aeruginosa and Bacillus subtillis strains was optimized using low-cost substrates. The highest biosurfactant production (3.2 g/L) by the P. aeruginosa strain was obtained using a culture medium containing corn steep liquor (CSL) (10% (v/v)) and molasses (10% (w/v)), whereas the best biosurfactant production by the B. subtillis isolate (1.3 g/L) was obtained using a culture medium consisting of 10% (v/v) of CSL. Subsequently, for the B. subtillis strain, the effect of different metals (iron, manganese and magnesium) on biosurfactant production was evaluated. When the culture medium CSL 10% was supplemented with the optimum concentration of those metals simultaneously, the biosurfactant production was increased up to 4.8 g/L. The biosurfactant produced by the P. aeruginosa strain was characterized as a mixture of eight different rhamnolipid congeners, being the most abundant the mono-rhamnolipid Rha-C10-C10, and the biosurfactant produced by the B. subtillis isolate consisted of a mixture of C13-, C14- and C15-surfactin. Both biosurfactants exhibited a good performance in oil recovery assays when compared with chemical surfactants, suggesting their potential use as an alternative to traditional chemical surfactants in enhanced oil recovery or bioremediation.
TypeConference paper
Publisher version
AccessOpen access
Appears in Collections:CEB - Artigos em Livros de Atas / Papers in Proceedings

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