Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/37622

TitlePeptide anchor for folate-targeted liposomal delivery
Author(s)Nogueira, E.
Mangialavori, Irene C.
Loureiro, Ana
Azóia, Nuno G.
Sarria, Marisa P.
Nogueira, Patrícia
Freitas, Jaime
Harmark, Johan
Shimanovich, Ulyana
Rollett, Alexandra
Lacroix, Ghislaine
Bernardes, Gonçalo J. L.
Guebitz, Georg
Hebert, Hans
Moreira, Alexandra
Carmo, Alexandre M.
Rossi, Juan Pablo F. C.
Gomes, Andreia
Preto, Ana
Cavaco-Paulo, Artur
Issue date4-Aug-2015
PublisherACS Publications
JournalBiomacromolecules
CitationNogueira, E.; Irene C. Mangialavori; Loureiro, A.; Azoia, Nuno G.; Passos, M.; Patrícia Nogueira; Jaime Freitas; Harmark, J.; Ulyana Shimanovich; Ulyana Shimanovich; Alexandra Rollett; Ghislaine Lacroix; Bernardes, Gonçalo J. L.; Guebitz, Georg; Hebert, H.; Alexandra Moreira; Alexandre M. Carmo; Juan Pablo F. C. Rossi; Andreia Gomes; Ana Preto; Paulo, Artur Cavaco, Peptide anchor for folate-targeted liposomal delivery. Biomacromolecules, 16(9), 2904-2910, 2015
Abstract(s)Specific folate receptors are abundantly overexpressed in chronically activated macrophages and in most cancer cells. Directed folate receptor targeting using liposomes is usually achieved using folate linked to a phospholipid or cholesterol anchor. This link is formed using a large spacer like polyethylene glycol. Here, we report an innovative strategy for targeted liposome delivery that uses a hydrophobic fragment of surfactant protein D linked to folate. Our proposed spacer is a small 4 amino acid residue linker. The peptide conjugate inserts deeply into the lipid bilayer without affecting liposomal integrity, with high stability and specificity. To compare the drug delivery potential of both liposomal targeting systems, we encapsulated the nuclear dye Hoechst 34580. The eventual increase in blue fluorescence would only be detectable upon liposome disruption, leading to specific binding of this dye to DNA. Our delivery system was proven to be more efficient (2-fold) in Caco-2 cells than classic systems where the folate moiety is linked to liposomes by polyethylene glycol.
TypeArticle
URIhttp://hdl.handle.net/1822/37622
ISBN1526-4602
DOI10.1021/acs.biomac.5b00823
ISSN1525-7797
e-ISSN1526-4602
Publisher versionhttp://pubs.acs.org/journal/bomaf6
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series
DBio - Artigos/Papers

Files in This Item:
File Description SizeFormat 
document_22388_1.pdf2,38 MBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID