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|Title:||Size controlled protein nanoemulsions for active targeting of folate receptor positive cells|
|Author(s):||Loureiro, Ana Isabel Sá|
Nogueira, Eugénia Sofia Costa
Azóia, Nuno G.
Sárria, M. P.
Abreu, Ana S.
Bernardes, Gonçalo J. L.
Gomes, Andreia C.
High pressure homogenization
|Journal:||Colloids and Surfaces B: Biointerfaces|
|Citation:||Loureiro, A.; Nogueira, E.; Azoia, Nuno G.; Passos, M.; Abreu, Ana S.; Shimanovich, Ulyana; Rollett, A.; Härmark, Johan; Hebert, Hans; Guebitz, Georg; Bernardes, Gonçalo J. L.; Preto, Ana; Gomes, Andreia C.; Paulo, Artur Cavaco, Size controlled protein nanoemulsions for active targeting of folate receptor positive cells. Colloids and Surfaces B: Biointerfaces, 135, 90-98, 2015|
|Abstract(s):||Bovine Serum Albumin (BSA) nanoemulsions were produced by high pressure homogenization with a tri-block copolymer (Poloxamer 407), which presents a central hydrophobic chain of polyoxypropylene (PPO) and two identical lateral hydrophilic chains of polyethylene glycol (PEG). We observed a linear correlation between tri-block copolymer concentration and size - the use of 5 mg/mL of Poloxamer 407 yields nanoemulsions smaller than 100 nm. Molecular dynamics and fluorescent tagging of the tri-block copolymer highlight their mechanistic role on the size of emulsions. This novel method enables the fabrication of highly stable albumin emulsions in the nano-size range, highly desirable for controlled drug delivery. Folic Acid (FA)-tagged protein nanoemulsions were shown to promote specific Folate Receptor (FR)-mediated targeting in FR positive cells. The novel strategy presented here enables the construction of size controlled, functionalized protein-based nanoemulsions with excellent characteristics for active targeting in cancer therapy.|
|Appears in Collections:||CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series|
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