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TitleSize controlled protein nanoemulsions for active targeting of folate receptor positive cells
Author(s)Loureiro, Ana Isabel Sá
Nogueira, Eugénia Sofia Costa
Azóia, Nuno G.
Sárria, M. P.
Abreu, Ana S.
Shimanovich, Ulyana
Rollett, Alexandra
Härmark, Johan
Hebert, Hans
Guebitz, Georg
Bernardes, Gonçalo J. L.
Preto, Ana
Gomes, Andreia C.
Cavaco-Paulo, Artur
KeywordsProtein-based nanoemulsions
High pressure homogenization
PEGylated surfactant
Folic acid
Active targeting
Issue dateNov-2015
PublisherElsevier B.V.
JournalColloids and Surfaces B: Biointerfaces
CitationLoureiro, A.; Nogueira, E.; Azoia, Nuno G.; Passos, M.; Abreu, Ana S.; Shimanovich, Ulyana; Rollett, A.; Härmark, Johan; Hebert, Hans; Guebitz, Georg; Bernardes, Gonçalo J. L.; Preto, Ana; Gomes, Andreia C.; Paulo, Artur Cavaco, Size controlled protein nanoemulsions for active targeting of folate receptor positive cells. Colloids and Surfaces B: Biointerfaces, 135, 90-98, 2015
Abstract(s)Bovine Serum Albumin (BSA) nanoemulsions were produced by high pressure homogenization with a tri-block copolymer (Poloxamer 407), which presents a central hydrophobic chain of polyoxypropylene (PPO) and two identical lateral hydrophilic chains of polyethylene glycol (PEG). We observed a linear correlation between tri-block copolymer concentration and size - the use of 5 mg/mL of Poloxamer 407 yields nanoemulsions smaller than 100 nm. Molecular dynamics and fluorescent tagging of the tri-block copolymer highlight their mechanistic role on the size of emulsions. This novel method enables the fabrication of highly stable albumin emulsions in the nano-size range, highly desirable for controlled drug delivery. Folic Acid (FA)-tagged protein nanoemulsions were shown to promote specific Folate Receptor (FR)-mediated targeting in FR positive cells. The novel strategy presented here enables the construction of size controlled, functionalized protein-based nanoemulsions with excellent characteristics for active targeting in cancer therapy.
Publisher version
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series
DBio - Artigos/Papers

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