Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/37013

TítuloThe effect of magnetic stimulation on the osteogenic and chondrogenic differentiation of human stem cells derived from the adipose tissue (hASCs)
AutorLima, João
Gonçalves, A. I.
Rodrigues, Márcia T.
Reis, R. L.
Gomes, Manuela E.
Palavras-chaveAdipose-derived stem cells
Chondrogenic differentiation
Magnetic nanoparticles
Osteogenic differentiation
Regenerative medicine
Tissue engineering
Data2015
EditoraElsevier
CitaçãoLima J., Gonçalves A. I., Rodrigues M. T., Reis R. L., Gomes M. E. The effect of magnetic stimulation on the osteogenic and chondrogenic differentiation of human stem cells derived from the adipose tissue (hASCs), Journal of Magnetism and Magnetic Materials , Vol. 393, pp. 526–536, doi:10.1016/j.jmmm.2015.05.087 , 2015
ResumoThe use of magnetic nanoparticles (MNPs) towards the musculoskeletal tissues has been the focus of many studies, regarding MNPs ability to promote and direct cellular stimulation and orient tissue responses. This is thought to be mainly achieved by mechano-responsive pathways, which can induce changes in cell behavior, including the processes of proliferation and differentiation, in response to external mechanical stimuli. Thus, the application of MNP-based strategies in tissue engineering may hold potential to propose novel solutions for cell therapy on bone and cartilage strategies to accomplish tissue regeneration. The present work aims at studying the influence of MNPs on the osteogenic and chondrogenic differentiation of human adipose derived stem cells (hASCs). MNPs were incorporated in hASCs and cultured in medium supplemented for osteogenic and chondrogenic differentiation. Cultures were maintained up to 28 days with/without an external magnetic stimulus provided by a magnetic bioreactor, to determine if the MNPs alone could affect the osteogenic or chondrogenic phenotype of the hASCs. Results indicate that the incorporation of MNPs does not negatively affect the viability nor the proliferation of hASCs. Furthermore, Alizarin Red staining evidences an enhancement in extracellular (ECM) mineralization under the influence of an external magnetic field. Although not as evident as for osteogenic differentiation, Toluidine blue and Safranin-O stainings also suggest the presence of a cartilage-like ECM with glycosaminoglycans and proteoglycans under the magnetic stimulus provided. Thus, MNPs incorporated in hASCs under the influence of an external magnetic field have the potential to induce differentiation towards the osteogenic and chondrogenic lineages.
Tipoarticle
URIhttp://hdl.handle.net/1822/37013
DOI10.1016/j.jmmm.2015.05.087
ISSN0304-8853
Versão da editorahttp://ac.els-cdn.com/S0304885315302109/1-s2.0-S0304885315302109-main.pdf?_tid=57cb53e4-2a20-11e5-9844-00000aab0f27&acdnat=1436875590_8bf49bb0a1a068cb1de2f90acafb4537
Arbitragem científicayes
AcessoopenAccess
Aparece nas coleções:3B’s - Artigos em revistas/Papers in scientific journals

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