Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/36605

TitleAdditively manufactured device for dynamic culture of large arrays of 3D tissue engineered constructs
Author(s)Costa, P. F.
Hutmacher, D. W.
Theodoropoulos, Christina
Gomes, Manuela E.
Reis, R. L.
Vaquette, C.
KeywordsAdditive manufacturing
Bioreactors
Regenerative medicine
Tissue engineering
Issue dateApr-2015
PublisherWiley-VCH Verlag
JournalAdvanced Healthcare Materials
CitationCosta P. F., Hutmacher D. W., Theodoropoulos C., Gomes M. E., Reis R. L., Vaquette C. Additively manufactured device for dynamic culture of large arrays of 3D tissue engineered constructs, Advanced Healthcare Materials, Vol. 4, Issue 6, pp. 864-873, doi:10.1002/adhm.201400591, 2015
Abstract(s)The ability to test large arrays of cell and biomaterial combinations in 3D environments is still rather limited in the context of tissue engineering and regenerative medicine. This limitation can be generally addressed by employing highly automated and reproducible methodologies. This study reports on the development of a highly versatile and upscalable method based on additive manufacturing for the fabrication of arrays of scaffolds, which are enclosed into individualized perfusion chambers. Devices containing eight scaffolds and their corresponding bioreactor chambers are simultaneously fabricated utilizing a dual extrusion additive manufacturing system. To demonstrate the versatility of the concept, the scaffolds, while enclosed into the device, are subsequently surface-coated with a biomimetic calcium phosphate layer by perfusion with simulated body fluid solution. 96 scaffolds are simultaneously seeded and cultured with human osteoblasts under highly controlled bidirectional perfusion dynamic conditions over 4 weeks. Both coated and noncoated resulting scaffolds show homogeneous cell distribution and high cell viability throughout the 4 weeks culture period and CaP-coated scaffolds result in a significantly increased cell number. The methodology developed in this work exemplifies the applicability of additive manufacturing as a tool for further automation of studies in the field of tissue engineering and regenerative medicine.
TypeArticle
URIhttp://hdl.handle.net/1822/36605
DOI10.1002/adhm.201400591
ISSN2192-2659
Peer-Reviewedyes
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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