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|Title: ||Establishment of a continuous model system to study Helicobacter pylori survival in potable water biofilms|
|Authors: ||Azevedo, N. F.|
Vieira, M. J.
Keevil, C. W.
|Keywords: ||Episcopic differential interference contrast microscopy|
Peptide nucleic acids
|Issue date: ||2002|
|Citation: ||INTERNATIONAL SPECIALISED CONFERENCE ON BIOFILM MONITORING, Porto, 2002 - "International Specialised Conference on Biofilm Monitoring, I.W.A". [S.l. : s.n.], 2002. p. 112-114.|
|Abstract: ||Close association of the pathogen Helicobacter pylori in drinking water biofilms has been suggested. Using a twostage
water model, the survival and development of the pathogen in potable water biofilms was monitored. Filter-sterilized tap
water was used as the growth medium and the inoculum consisted of a naturally occurring consortium of microorganisms.
Biofilms were generated on removable stainless steel coupons that were placed in the second vessel. Novel technology peptide
nucleic acid (PNA) molecular probes were used to detect and locate the pathogen within the biofilms which were also stained
with the vital fluorophore, CTC, as a measure of viability. The PNA-labelled oligonucleotide probes were highly specific, and
complementary to the helix 6 region of H. pylori 16S rRNA. The pathogen was tracked in the biofilms using epifluorescence
microscopy (EF) and episcopic differential interference contrast microscopy (EDIC).|
|Appears in Collections:||CEB - Artigos em Livros de Atas / Papers in Proceedings|
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