Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/36328
Título: | Hydrogenotrophic activity under increased H₂/CO₂ pressure: effect on methane production and microbial community |
Autor(es): | Lopes, Marlene Alves, J. I. Pereira, A. Belo, Isabel Sousa, D. Z. Alves, M. M. |
Data: | 20-Ago-2015 |
Editora: | Elsevier B.V. |
Revista: | Journal of Biotechnology |
Resumo(s): | H2 and CO2 are main compounds of synthesis gas. Efficient conversion of syngas to biomethane is a straightforward strategy to integrate the energy value of syngas into existing natural gas grid infrastructures. In this study, the effect of initial H2/CO2 (80/20, v/v) pressure on methane production rate and microbial community diversity was assessed in a hyperbaric bioreactor inoculated with anaerobic granular sludge. Several batch experiments were performed to distinguish between the effect of initial total gas pressure and H2/CO2 partial pressure: (1) varying initial gas pressure (from 1 to 6 bar) with 100% H2/CO2 mixture; (2) constant initial gas pressure (5 bar), with increasing H2/CO2 partial pressure (from 1 to 5 bar); (3) varying initial gas pressure (from 2 to 5 bar) with constant H2/CO2 partial pressure (2 bar). In (2) and (3), N2 was used for ensuring the necessary overpressure. Microbial community changes in the system were monitored by 16S rRNA-based techniques (PCR-DGGE). The raise of H2/CO2 initial pressure (100% H2/CO2) from 1 to 5 bar led to an improvement in methane rate production from 0.035 ± 0.014 mmol h1 to 0.072 ± 0.019 mmol h1. Similar methane production rates were observed in reactors operated at the same H2/CO2 partial pressures, even when varying the total initial gas pressure. Hydrogen partial pressure was shown to determine the structure of bacterial communities and diversity decreased with increasing H2/CO2 partial pressure. No significant changes were observed for the archaeal communities. |
Tipo: | Resumo em ata de conferência |
URI: | https://hdl.handle.net/1822/36328 |
DOI: | 10.1016/j.jbiotec.2015.06.170 |
ISSN: | 0168-1656 |
e-ISSN: | 0168-1656 |
Versão da editora: | http://www.elsevier.com/locate/issn/01681656 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_22251_1.pdf | 79,43 kB | Adobe PDF | Ver/Abrir |